Human SCF/c-kit Ligand ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1022

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernatant, Plasma, Serum (50 uL)

Sensitivity

0.09 ng/mL

Assay Range

0.156 to 40 ng/mL

View all SCF/c-kit Ligand ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

KITLG (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human KITLG in plasma, serum, and cell culture supernatants.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.50%

Inter-Assay Precision (Precision between assays) CV% < 7.20%

Recovery for Human SCF/c-kit Ligand ELISA Kit (Colorimetric)

Recovery

96%

Scientific Data Images for Human SCF/c-kit Ligand ELISA Kit (Colorimetric)

ELISA: Human SCF/c-kit Ligand ELISA Kit (Colorimetric) [KA1022]

ELISA: Human SCF/c-kit Ligand ELISA Kit (Colorimetric) [KA1022]

ELISA: SCF/c-kit Ligand ELISA Kit [HRP] [KA1022] - Standard curve

Kit Contents for Human SCF/c-kit Ligand ELISA Kit (Colorimetric)

  • Biotinylated SCF Antibody (100x): A 100-fold concentrated biotinylated polyclonal antibody against SCF.
  • Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine.
  • EIA Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
  • Human SCF Microplate: A polystyrene microplate coated with a polyclonal antibody against human SCF.
  • Human SCF Standard: Human SCF in a buffered protein base (lyophilized).
  • Sealing Tapes: Each kit contains pre-cut, pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay.
  • Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction.
  • Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.
  • Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: SCF/c-kit Ligand

Mouse SCF (Stem cell factor, steel factor, Kit ligand, C-kit-ligand) is a hematopoietic growth factor that stimulates the proliferation of bone marrow stem cells. SCF induces the proliferation of mast cells and primitive lymphoid and myeloid hematopoietic bone marrow progenitor cells in culture. SCF acts synergistically with both GM-CSF and G-CSF, IL-7 and EPO. Mouse SCF is active on human cells as well as murine cells and can be used for long term bone marrow culture.

Long Name

Stem Cell Factor

Alternate Names

c-kit Ligand, DCUA, DFNA69, FPH2, FPHH, KITLG, KL-1, MGF, SHEP7, SLF

Gene Symbol

KITLG

Additional SCF/c-kit Ligand Products

Product Documents for Human SCF/c-kit Ligand ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human SCF/c-kit Ligand ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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