Human Serpin E1/PAI-1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83740

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution (50 uL)

Sensitivity

29.0 pg/mL

Assay Range

78 - 5,000 pg/mL

View all Serpin E1/PAI-1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no crossreactivity detected.

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.7%

Inter-Assay Precision (Precision between assays) CV% < 5.0%

Recovery for Human Serpin E1/PAI-1 ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 64.5% to 84.8% with an overall mean recovery of 72.2% for serum and from 44.8% to 68.4%with an overall mean recovery of 58.7% for plasma samples.

Scientific Data Images for Human Serpin E1/PAI-1 ELISA Kit (Colorimetric)

ELISA: Human Serpin E1/PAI-1 ELISA Kit (Colorimetric) [NBP1-83740]

ELISA: Human Serpin E1/PAI-1 ELISA Kit (Colorimetric) [NBP1-83740]

ELISA: Human Serpin E1/PAI-1 ELISA Kit (Colorimetric) [NBP1-83740] - Representative standard curve for human PAI-1 ELISA. Human PAI-1 was diluted in serial 2-fold steps in AssayBuffer (1x). Do not use this standard curve to derive test results. A standard curve must be run for each group ofmicrowell strips assayed.

Kit Contents for Human Serpin E1/PAI-1 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human PAI-1
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human PAI-1 polyclonal antibody
  • Human PAI-1 Standard lyophilized, 10000 pg/mL upon reconstitution
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Serpin E1/PAI-1

Plasminogen activator inhibitor-1 (PAI-1) is the primary inhibitor of plasminogen activators in plasma, rapidly inactivating both tissue plasminogen activator (t-PA) and urokinase-type plasminogen activator (u-PA). PAI-1 is a single-chain glycoprotein with a molecular weight of 47 kilodaltons. During fibrinolysis, tissue plasminogen activator (tPA) converts the inactive protein plasminogen into plasmin which plays a critical role in fibrinolysis by degrading fibrin and providing localized protease activity in a number of physiological functions. PAI-1 is synthesized in the liver and by endothelial cells, and its synthesis is regulated by several physiologic mediators, including endotoxin, interleukin-1, fibroblast growth factor-2, and lipids. Plasminogen activator inhibitor-1 is an important inhibitor of the fibrinolytic system, so elevated levels could suppress fibrinolysis and result in an increased risk of thrombosis. Increased PAI-1 levels have been shown to be associated with a number of atherosclerotic risk factors, PAI-1 has been shown to act as a prothrombic factor in both arterial and venous thromboembolic disorders. Increased levels of PAI-1 are associated with an increased incidence of acute coronary syndrome. PAI-1 levels are also increased in patients with chronic and acute coronary artery disease (CAD) and in patients who suffer restenosis after coronary angioplasty.

Long Name

Plasminogen Activator Inhibitor

Alternate Names

Nexin, PAI-1, PAI1, PLANH1

Gene Symbol

SERPINE1

Additional Serpin E1/PAI-1 Products

Product Documents for Human Serpin E1/PAI-1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Serpin E1/PAI-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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