Rat Serpin E1/PAI-1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18763

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.94 ng/mL

Assay Range

1.56-100 ng/mL

View all Serpin E1/PAI-1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Serpin E1/PAI-1 in samples. No significant cross-reactivity or interference between Rat Serpin E1/PAI-1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.64

Inter-Assay Precision (Precision between assays) %CV < 5.07

Recovery for Rat Serpin E1/PAI-1 ELISA Kit (Colorimetric)

Recovery

85-105%

Linearity

Rat Serpin E1/PAI-1 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Serpin E1/PAI-1 ELISA Kit (Colorimetric)

Rat Serpin E1/PAI-1 ELISA Kit (Colorimetric)

ELISA: Rat Serpin E1/PAI-1 ELISA Kit (Colorimetric) [NBP3-18763] -

ELISA: Rat Serpin E1/PAI-1 ELISA Kit (Colorimetric) [NBP3-18763] - Standard Curve Reference

Kit Contents for Rat Serpin E1/PAI-1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Serpin E1/PAI-1

PAI-1 and PAI-2 (for plasminogen activator inhibitor-1 and -2) are members of the serpin serine proteinase inhibitor family. PAI-1 and PAI-2 have been shown to regulate uPA (urokinase-type plasminogen activator) and tPA (tissue plasminogen activator), resulting in the inhibition of proteolytic activity. Members of the serpin family generally complex with their target proteinases, then disassociate slowly into cleaved species that fold into stable inactive forms. PAI-1 can fold into the inactive state without cleavage, resulting in the latent form of PAI-1. Activity can be restored to the latent form of PAI-1 through denaturation and renaturation. PAI-2 occurs in secreted and cytosolic forms through facultative polypeptide translocation. uPA is a serine proteinase that is a member of the trypsin family. It is responsible for the cleavage of plasminogen at the Arg-Val bond to produce plasmin. uPA consists of two chains designated A and B. The A chain can be cleaved, resulting in low and high molecular mass forms of uPA.

Long Name

Plasminogen Activator Inhibitor

Alternate Names

Nexin, PAI-1, PAI1, PLANH1

Gene Symbol

SERPINE1

Additional Serpin E1/PAI-1 Products

Product Documents for Rat Serpin E1/PAI-1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Serpin E1/PAI-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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