Human SOD1/Cu-Zn SOD ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75920

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), amniotic fluid, urine, fetal umbilical vein blood (10 uL)

Sensitivity

0.04 ng/mL

Assay Range

0.08 - 5.0 ng/mL

View all SOD1/Cu-Zn SOD ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human Cu/ZnSOD positive serum. There was no crossreactivity detected.

Description

The human Cu/ZnSOD ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Cu/ZnSOD

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.1%

Inter-Assay Precision (Precision between assays) CV% < 5.8%

Recovery for Human SOD1/Cu-Zn SOD ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 89% to 108% with an overall mean recovery of 98%. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Human SOD1/Cu-Zn SOD ELISA Kit (Colorimetric)

ELISA: Human SOD1/Cu-Zn SOD ELISA Kit (Colorimetric) [NBP2-75920]

ELISA: Human SOD1/Cu-Zn SOD ELISA Kit (Colorimetric) [NBP2-75920]

ELISA: Human SOD1/Cu-Zn SOD ELISA Kit (Colorimetric) [NBP2-75920] - Standard curve

Kit Contents for Human SOD1/Cu-Zn SOD ELISA Kit (Colorimetric)

  • 1 vial (60 ul) human Cu/ZnSOD Standard protein 400ng/ml
  • 1 vial (2.75ml) monoclonal Coating Antibody to human Cu/ZnSOD (100 ug/ml)
  • 1 vial (55 ul) HRP-Conjugate anti-human Cu/ZnSOD monoclonal antibody

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: SOD1/Cu-Zn SOD

Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3). SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80 kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin binding multimer of disulfide linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperon for superoxide dismutase(CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor. Superoxide dismutase recognizes copper/zinc superoxide dismutase (SOD). Superoxide dismutases are ubiquitous metalloproteins that destroy oxygen-mediated free radicals, that are normally produced within the cells, which are toxic to biological systems. There are three forms of superoxide dismutase, including the Fe, mitochondrial (Mn) and the copper/zinc binding (Cu/Zn) form. The Cu/Zn form of SOD is utilised by most eukaryotic organisms. SOD Cu/Zn prevents oxygen-mediated free radical damage by catalysing the dismutation of the toxic superoxide radical to molecular oxygen and hydrogen peroxide.

Long Name

Superoxide Dismutase-1

Alternate Names

Cu-Zn SOD, CuZn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble

Gene Symbol

SOD1

UniProt

P00441 (Human)

Additional SOD1/Cu-Zn SOD Products

Product Documents for Human SOD1/Cu-Zn SOD ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human SOD1/Cu-Zn SOD ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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