Substance P Parameter Assay Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # KGE007
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), Heparin Plasma (50 µL), Saliva (50 µL), Urine (50 µL)
Sensitivity
43.8 pg/mL
Assay Range
39-2500 pg/mL (Cell Culture Supernates)
Product Summary for Substance P Parameter Assay Kit
The Parameter Substance P Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure Substance P in cell culture supernates, serum, plasma, saliva, and urine. It contains a synthetically derived human Substance P peptide and has been shown to accurately quantitate this peptide. Results obtained using samples containing natural Substance P showed linear curves that were parallel to the standard curves obtained using the Parameter kit standards. These results indicate that this kit can be used to determine relative mass values for natural Substance P.
Product Specifications
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
Biotin
Reactivity
Multi-Species
Specificity
Measures Substance P levels in various samples
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Four samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Four samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||||
|---|---|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 |
| n | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 146 | 258 | 616 | 1973 | 140 | 262 | 684 | 1923 |
| Standard Deviation | 12.3 | 15.1 | 21.5 | 142 | 18.1 | 26.8 | 103 | 180 |
| CV% | 8.4 | 5.8 | 3.5 | 7.2 | 12.9 | 10.2 | 15.0 | 9.3 |
Recovery for Substance P Parameter Assay Kit
The recovery of Substance P spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 94 | 82-110 |
| Heparin Plasma (n=4) | 100 | 85-116 |
| Saliva (n=4) | 102 | 90-110 |
| Serum (n=4) | 101 | 85-117 |
| Urine (n=4) | 89 | 76-107 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Substance P were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Substance P Parameter Assay Kit
Multi-species Substance P ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Substance P
Additional Substance P Products
Product Documents for Substance P Parameter Assay Kit
Product Specific Notices for Substance P Parameter Assay Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.