Tankyrase 1 Chemiluminescent Activity Assay

The Tankyrase 1 Chemiluminescent Activity Assay is a high-throughput, semi-quantitative ELISA designed to measure Tankyrase 1 activity. Tankyrase 1, also known as PARP5a, is a Poly (ADP-ribose) Polymerase (PARP) that associates with telomeres via its interaction with the telomeric-repeat binding factor TRF1. This assay utilizes a histone-coated 96-well microplate and an anti-poly (ADP-ribose) (PAR) antibody to detect PAR that has been deposited onto the immobilized histone proteins by Tankyrase 1. The Tankyrase 1 Chemiluminescent Activity Assay uses PARP PeroxyGlow™ A and B. This is the only commercially available assay to feature a full length Tankyrase 1 enzyme and unmodified NAD substrate to create a more physiologically applicable in vitro assay system.

Features

• Amenable to high-throughput
• Highly sensitive: capable of measuring 0.1 mUnits of Tankyrase 1
• 3 hour assay time
• Provides full length Tankyrase 1 enzyme and unmodified NAD substrate

Kit Contents

• Histone-coated 96-stripwell Microplate
• Anti-PAR Monoclonal Antibody
• HRP-conjugated Goat Anti-Mouse Antibody
• Tankyrase 1
• XAV939^a
• Diluents & Buffers
• Unmodified NAD Substrate
• HRP Substrate

Data Example

Inhibition of Tankyrase 1 Activity by XAV939

Graphical representation of a chemiluminescent Tankyrase 1 standard curve (A) and an inhibition curve for the Tankyrase 1 inhibitor XAV939 (provided in the kit; B). The standard curve is used to translate absorbance values to activity units of Tankyrase 1.

^aXAV939 is a Tankyrase 1 inhibitor TACS-Sapphire and PeroxyGlow are trademarks of Trevigen, Inc.