Human Thrombospondin-1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60149

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, breast milk, saliva, tissue homogenates and cell culture supernates (50 - 100 uL)

Sensitivity

2.7 ng/mL

Assay Range

2.7 ng/mL - 2000 ng/mL

View all Thrombospondin-1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human TSP-1. No significant cross-reactivity or interference between human TSP-1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Scientific Data Images for Human Thrombospondin-1 ELISA Kit (Colorimetric)

ELISA: Human Thrombospondin-1 ELISA Kit (Colorimetric) [NBP2-60149]

ELISA: Human Thrombospondin-1 ELISA Kit (Colorimetric) [NBP2-60149]

ELISA: Human Thrombospondin-1 ELISA Kit (Colorimetric) [NBP2-60149] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Human Thrombospondin-1 ELISA Kit (Colorimetric)

Human Thrombospondin-1 ELISA Kit (Colorimetric) [NBP2-60149] -

Human Thrombospondin-1 ELISA Kit (Colorimetric) [NBP2-60149] - Standard Curve Reference

Kit Contents for Human Thrombospondin-1 ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate (12 x 8 coated Microwells)
  • Biotin-antibody (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: Thrombospondin-1

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TSP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Alternate Names

THBS1, Thrombospondin1, TSP-1

Gene Symbol

THBS1

Additional Thrombospondin-1 Products

Product Documents for Human Thrombospondin-1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Thrombospondin-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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