Human TNF RII/TNFRSF1B ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88105

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), urine (10 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10.0 ng/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This antibody targets the soluble protein. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human TNF-R (80 kDa) positive serum. There was no crossreactivity detected, namely not with TNF alpha, TNF bata and TNF-R (60 kDa).

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 1.4%

Inter-Assay Precision (Precision between assays) CV% < 2.0%

Recovery for Human TNF RII/TNFRSF1B ELISA Kit (Colorimetric)

Recovery

The amount of endogenous human TNF-R (80 kDa) in unspiked serum was subtracted from the spike values. The recovery ranged from 87% to 109% with an overall mean recovery of 94%.

Scientific Data Images for Human TNF RII/TNFRSF1B ELISA Kit (Colorimetric)

ELISA: Human TNF RII/TNFRSF1B ELISA Kit (Colorimetric) [NBP1-88105]

ELISA: Human TNF RII/TNFRSF1B ELISA Kit (Colorimetric) [NBP1-88105]

ELISA: Human TNF RII/TNFRSF1B ELISA Kit (Colorimetric) [NBP1-88105] - Representative standard curve for human TNF-RII (80 kDa) ELISA. Human TNF-RII (80 kDa) was diluted in serial 2-fold steps in Assay Buffer (1x). Do not use this standard curve to derive test results. A standard curve must be run foreach group of microwell strips assayed.

Kit Contents for Human TNF RII/TNFRSF1B ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sTNF-R (80 kDa)
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Control, lyophilized
  • HRP-Conjugate anti-human sTNF-R (80 kDa) monoclonal antibody
  • Human sTNF-R (80 kDa) Standard, 10 ng/ml
  • Stop Solution (1M Phosphoric acid)
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TNF RII/TNFRSF1B

Tumor Necrosis Factor (TNF) was originally discovered in sera of animals and was found to cause hemorrhagic necrosis of some transplantable mouse and human tumors and to exhibit primarily cytotoxic activities against tumor but not normal cells in vitro. The TNF family consists of two proteins designated TNF alpha, also called cachectin, and TNF beta, also called lymphotoxin, which are pleiotropic cytokines that can mediate a wide variety of biological effects. Both TNF alpha and TNF beta have been shown to interact with a cell through specific high affinity receptors with a few hundred up to more than 20,000 copies per cell. TNF-receptors have been demonstrated on a wide variety of human somatic cells including fibroblasts, endothelial cells, adipocytes, liver membranes, granulocytes and several tumor cell lines. Normal and malignant human myeloid cells as well as mitogen- stimulated lymphocytes express similar numbers of TNF receptors (400-1,900 per cell), whereas resting lymphoid cells have fewer, red blood cells and platelets have no detectable TNF receptors. In most cases no correlation is observed between receptor number and sensitivity to TNF. Based on gel filtration experiments the receptor appears to be a complex of different proteins with a molecular weight of 350 kDa. In a variety of cell lines two different types of TNF receptors with 75 - 80 and 55 - 60 kDa respectively have been identified. The cDNAs encoding the two different TNF receptors have been cloned. The predicted amino acid sequences of the extracellular regions of the two TNF-R reveal significant similarities. The 60 kDa receptor consists of 426 amino acids with a single membrane span, an extracellular domain of 182 amino acids and an intracellular domain of 223 amino acids. The 80 kDa receptor is also a single membrane-spanning receptor of 439 amino acids with an extracellular domain of 235 amino acids and an intracellular domain of 178 amino acids. They share 28 % identity on their extracellular domain, but their intracellular parts are totally different from each other

Long Name

Tumor Necrosis Factor Receptor II

Alternate Names

CD120b, TNFRII, TNFRSF1B

Gene Symbol

TNFRSF1B

Additional TNF RII/TNFRSF1B Products

Product Documents for Human TNF RII/TNFRSF1B ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human TNF RII/TNFRSF1B ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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