Human, Canine Transferrin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61298

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

human serum, human plasma and canine serum (100 uL)

Sensitivity

4.6 ng/ml

Assay Range

4.9 - 5000 ng/ml

View all Transferrin ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human, Canine

Precision

Intra-Assay Precision (Precision within an assay) Mean 6.4%

Inter-Assay Precision (Precision between assays) Mean 14.1%

Recovery for Human, Canine Transferrin ELISA Kit (Colorimetric)

Recovery

Human Serum - 83%
Human EDTA Plasma - 110%
Canine Serum - 143%

Scientific Data Images for Human, Canine Transferrin ELISA Kit (Colorimetric)

ELISA: Human, Canine Transferrin ELISA Kit (Colorimetric) [NBP2-61298]

ELISA: Human, Canine Transferrin ELISA Kit (Colorimetric) [NBP2-61298]

ELISA: Transferrin ELISA Kit (Colorimetric) [NBP2-61298] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Human, Canine Transferrin ELISA Kit (Colorimetric)

  • Antibody
  • Antibody Diluent
  • Assay Buffer 13
  • Conjugate
  • Microtiter Plate
  • Standard
  • Stop Solution
  • Substrate
  • Tracer
  • Wash Buffer Concentrate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: Transferrin

Human serum transferrin, one of the members of the family of transferrin proteins, is an 80kDa glycoprotein and is a major carrier of iron in blood. Also included in this family of proteins are: i) lactoferrin, which is the iron-binding protein found in human milk and ii) ovotransferrin, which is the iron-binding protein isolated from avian egg white. Transferrin binds iron, forming a transferrin-iron complex. When this complex encounters a transferrin receptor on the surface of the cell, it binds and is transported into the cytoplasm. Once inside the cell, the iron is released and the iron- free transferrin, now referred to as apotransferrin, is transported back to the cell surface. Each molecule of transferrin can bind up to two atoms of iron to form reddish protein-iron complexes. Transferrin allows for the transport of the ferric iron (Fe3+) from the intestine, reticuloendothelial system and liver parenchymal cells to all cells in the body. Measurements of serum transferrin has applications in anemia, where medical professionals may check transferrin levels in iron deficiency, as an increase in transferrin levels may be seen in patients with iron deficiency anemia. Additionally, transferrin has a role in iron overload disorders such as hemochromatosis, where transferrin levels would be decreased in plasma and serum. Decreased levels of transferrin can also be detected in patients with liver diseases, such as cirrhosis and non-alcoholic fatty liver disease.

Alternate Names

Beta-1 metal-binding globulin, Serotransferrin, Siderophilin, TF

Gene Symbol

TF

Additional Transferrin Products

Product Documents for Human, Canine Transferrin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human, Canine Transferrin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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