Human VEGFR1/Flt-1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91265

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (citrate, EDTA), buffered solution (50 uL)

Sensitivity

0.03 ng/mL

Assay Range

0.16 - 10.0 ng/mL

View all VEGFR1/Flt-1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The assay detects both natural and recombinant human VEGF-R1. The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human VEGF-R1 positive sample. Interference was detected for VEGF-A at concentrations > 0.15 ng/ml and for PLGF-1 at concentrations > 2.5 ng/ml. There was no cross reactivity or interference detected for KDR, PDGFAA, PDGF-BB, VEGF-B, VEGF-C, VEGF-D, HGF, EGF.

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.50%

Inter-Assay Precision (Precision between assays) CV% < 5.10%

Recovery for Human VEGFR1/Flt-1 ELISA Kit (Colorimetric)

Recovery

For recovery data see the manual.

Scientific Data Images for Human VEGFR1/Flt-1 ELISA Kit (Colorimetric)

Human VEGFR1/Flt-1 ELISA Kit [NBP1-91265] - Data for Human sVEGF-R1 Platinum ELISA

Kit Contents for Human VEGFR1/Flt-1 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sVEGF-R1
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human sVEGF-R1 polyclonal antibody
  • Conjugate Diluent
  • Human sVEGF-R1 Standard lyophilized, 20 ng/mL upon reconstitution
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: VEGFR1/Flt-1

Soluble VEGF-R1 (sFLT-1) is a naturally occurring endogenous form of the VEGF-R1 and was originally found in the supernatant of human vascular endothelial cells. It is generated by differential splicing of the flt-1 gene. In vitro sVEGF-R1 is used to inhibit VEGF-A mediated signals in endothelial cells and in vivo it can be used to block physiological angiogenesis in several organs, e.g. in the ovary or in bones. Tumor cells transfected with the flt-1 gene are growth restricted in vivo because of the limitation in developing tumor blood vessels via VEGF-A signalling. Characterization of angiogenic activity, such as embryonic development, placental vascularization, cancer and wound healing is measured by comparing the ratio of angiogenic stimulators (e.g. FGF-2, FGF-1, VEGF-A, Ang-1) to angiogenic inhibitors (e.g. sFLT-1, angiostatin, endostatin, thrombospondin).

Long Name

Vascular Endothelial Growth Factor Receptor 1

Alternate Names

Flt-1, FLT1, FRT, VEGF R1, VEGFR-1

Gene Symbol

FLT1

Additional VEGFR1/Flt-1 Products

Product Documents for Human VEGFR1/Flt-1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human VEGFR1/Flt-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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