Rat VIP ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82466

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

4.69 pg/mL

Assay Range

7.81 - 500 pg/mL

View all VIP ELISA Kits »

Product Specifications

Assay Type

Competitive-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat VIP in samples. No Significant cross-reactivity or interference between Rat VIP and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.21

Inter-Assay Precision (Precision between assays) CV% < 4.78

Recovery for Rat VIP ELISA Kit (Colorimetric)

Recovery

86-106%

Linearity

Rat VIP ELISA Kit (Colorimetric)

Scientific Data Images for Rat VIP ELISA Kit (Colorimetric)

Rat VIP ELISA Kit (Colorimetric)

ELISA: Rat VIP ELISA Kit (Colorimetric) [NBP2-82466] -

ELISA: Rat VIP ELISA Kit (Colorimetric) [NBP2-82466] - Standard Curve Reference

Kit Contents for Rat VIP ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: VIP

Vasoactive intestinal peptide (VIP) is a 28-amino-acid peptide belonging to the secretin-glucagon family of peptide hormones. Its sequence is identical in man, ox, pig and rat. VIP is widely distributed in the central and peripheral nervous systems, acting as a neurotransmitter or neuromodulator. Peripherally it is involved in vaso- and bronchodilatation, smooth muscle relaxation and stimulation of secretion. Ectopic endocrine tumors secreting VIP (VIPomas) give rise to raised plasma levels of VIP and are associated with watery diarrhea, hypokalemia and achlorhydria syndrome. VIP shows high sequence homology with peptide histidine-methionine (PHM) in man or peptide histidine-serine (PHI) in pigs or rats, present in the same precursor, and with the neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP).

Long Name

Vasoactive Intestinal Peptide

Alternate Names

MGC13587

Gene Symbol

VIP

Additional VIP Products

Product Documents for Rat VIP ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat VIP ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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