Human WISP-1/CCN4 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00420

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-00420
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL

View all WISP-1/CCN4 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human WISP-1/CCN4 in samples. No significant cross-reactivity or interference between Human WISP-1/CCN4 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 5.12%

Inter-Assay Precision (Precision between assays) 5.46%

Recovery for Human WISP-1/CCN4 ELISA Kit (Colorimetric)

Recovery

87 - 106%

Linearity

Human WISP-1/CCN4 ELISA Kit (Colorimetric)

Scientific Data Images for Human WISP-1/CCN4 ELISA Kit (Colorimetric)

Human WISP-1/CCN4 ELISA Kit (Colorimetric)

ELISA: Human WISP-1/CCN4 ELISA Kit (Colorimetric) [NBP3-00420] -

ELISA: Human WISP-1/CCN4 ELISA Kit (Colorimetric) [NBP3-00420] - Standard Curve Reference

Kit Contents for Human WISP-1/CCN4 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: WISP-1/CCN4

WISP1 encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

Long Name

Wnt-1 Induced Secreted Protein 1

Alternate Names

CCN4, WISP1

Gene Symbol

CCN4

Additional WISP-1/CCN4 Products

Product Documents for Human WISP-1/CCN4 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human WISP-1/CCN4 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.