Mouse IL-17 ELISpot Kit

Name: Mouse IL-17 ELISpot Kit
Brand: R&D Systems
SKU: EL421

R&D Systems, part of Bio-Techne | Catalog # EL421

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Product Datasheet / COA / SDS

Key Product Details

Assay Type:	Quantitative Sandwich ELISA
Format:	96-well PVDF-backed microplate
Assay Length:	3 hours 15 mins to 4 hours 30 mins*
Sample Types:	Whole Cells
Specificity:	Please see the product datasheet

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Technical Data
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Product Summary for Mouse IL-17 ELISpot Kit

ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. This kit is designed for the detection and enumeration of mouse IL-17/IL-17A. Complete ELISpot kits are ready-to-run and require no assay development or refinement.

This ELISpot assay employs a capture antibody specific for mouse IL-17/IL-17A, pre-coated onto a PVDF-backed microplate. Appropriately stimulated cells are pipetted directly into the wells and the immobilized antibody in the immediate vicinity of the secreting cells binds secreted mouse IL-17/IL-17A. Following wash steps and incubation with a biotinylated detection antibody, alkaline-phosphatase conjugated to streptavidin is added. Unbound enzyme is subsequently removed by washing and a substrate solution (BCIP/NBT) is added. A blue-black colored precipitate forms at the sites of cytokine localization and appears as spots, with each individual spot representing an individual mouse IL-17/IL-17A secreting cell. The spots can be counted with an automated ELISpot reader system or manually using a stereomicroscope.

Product Features for Mouse IL-17 ELISpot Kit

Detect and quantitate individual cells secreting mouse IL-17/IL-17A
High sensitivity - ELISpot assays can measure responses with frequencies well below 1 in 100,000 cells
No in vitro expansion of cells required
High-throughput - ELISpot assays use only a small number of primary cells

Kit Contents for Mouse IL-17 ELISpot Kit

Mouse IL-17/IL-17A Microplate
Biotinylated Detection Antibody
Streptavidin conjugated to Alkaline Phosphatase
Dilution Buffers
Wash Buffer Concentrate
BCIP/NBT Chromogen
Mouse IL-17/IL-17A Positive Control

Other Reagents Required

Pipettes and pipette tips
Deionized or distilled water
Squirt bottle, manifold dispenser, or automated microplate washer
500 mL graduated cylinder
37 °C CO₂ incubator
Sterile culture media
Dissection microscope or an automated ELISpot reader

Formulation, Preparation and Storage

Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17/IL-17A

The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.

Item	Value
Long Name	Interleukin 17
Entrez Gene IDs	3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names	CTLA-8, CTLA8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL-17, IL-17A, IL-17Acytotoxic T-lymphocyte-associated protein 8, IL-17CTLA-8, IL17, IL17A, IL17interleukin-17A, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), interleukin 17A

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Product Documents for Mouse IL-17 ELISpot Kit

Product Datasheets

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COA

SDS

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⚠ WARNING: This product can expose you to chemicals including methanol, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.