Anti-Mouse Ig (H+L) Comp-Bead 3 Population (7.5 um) Kit

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00499

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-00499
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Applications

Flow Cytometry

Kit Type

Kit

Product Summary for Anti-Mouse Ig (H+L) Comp-Bead 3 Population (7.5 um) Kit

The Anti-Mouse Ig (H+L) Compensation Beads are designed to capture fluorophore-conjugated antibodies, serving as accurate and consistent compensation controls in multicolor flow cytometry. Bind to antibodies of mouse, rat, and hamster origin and are immunoglobulin light chain independent Compatible with UV- to near IR-excited fluorophores Exhibit low autofluorescence regardless of excitation wavelength (e.x. violet laser or red laser) or detection bandpass Contains 3 populations (negative, low, high) for comprehensive quality control of fluorophores Concentration: 2.5 x 10^6 particles / mL

Particle size: 7.0 -7.9 micron

Particle material: polystyrene beads coated with goat anti-mouse IgG

Scientific Data Images for Anti-Mouse Ig (H+L) Comp-Bead 3 Population (7.5 um) Kit

Anti-Mouse Ig (H+L) Comp-Bead 3 Population (7.5 um) Kit [NBP3-00499] - Histogram of flow cytometry data showing fron and side scatter and the three populations in the FITC channel. Dot plots show uncompensated and compensation populations in the FITC Log X PE Log.

Kit Contents for Anti-Mouse Ig (H+L) Comp-Bead 3 Population (7.5 um) Kit

  • Comp Beads (high) - 5 mL
  • Comp Beads (low) - 5 mL
  • Comp Beads (negative) - 5 mL

Formulation, Preparation, and Storage

Formulation

PBS, 0.2% BSA

Preservative

0.02% Sodium Azide

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C. Do not freeze.

Background: Anti-Mouse Ig (H+L) Comp-Bead 3 Population (7.5 um)

Compensation is the mathematical correction for the amount of overlap (spillover) of one fluorochrome's emission spectra into another fluorochrome's channel. To calculate this overlap, record single-color stained controls for each fluorochrome in a multicolor panel. This allows the cytometer to visualize the emission of each fluorochrome in each channel, or detector, and subtract the spillover, generating a matrix to apply to multi-color experimental samples. To ensure accurate calculations, single-color controls need to be as bright, or brighter, than the marker on the cell of interest. With increased use of multi-parametric flow cytometry and the large number of fluorochromes available, it is more important than ever to ensure accurate compensation for reliable interpretation of data and reproducible experimental results.

Compensation beads are a highly desirable alternative to traditional cell-based single-color compensation. Positive beads can come pre-loaded with anti-IgG for conjugated antibody capture of mouse, rat, or hamster origin. Uncoated (negative) beads ensure standardization of the autofluorescence in each channel. Beads are preferable to cell-based compensation for several reasons:

1) No precious sample is wasted, and more sample can be used for experimental acquisition.

2) Since 100% of positive beads are able to capture the antibody, the exact experimental fluorochrome can be used regardless of a marker's cellular expression. Dimly expressed markers may not be bright enough for compensation using cells.

3) Reduced autofluorescence of beads allows for more precise calculation of spectral overlap.

Alternate Names

Comp Beads, Compensation Beads, Compensation Capture Beads, Flow Cytometry Compensation

Additional Anti-Mouse Ig (H+L) Comp-Bead 3 Population (7.5 um) Products

Product Documents for Anti-Mouse Ig (H+L) Comp-Bead 3 Population (7.5 um) Kit

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Anti-Mouse Ig (H+L) Comp-Bead 3 Population (7.5 um) Kit

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.