Acridine Orange hydrochloride

Name: Acridine Orange hydrochloride
Brand: Tocris Bioscience
SKU: 5092

Tocris Bioscience, part of Bio-Techne | Catalog # 5092

COA / SDS

Nucleic acid binding dye. Cell permeable. Used for: cell cycle and apoptosis determination. Application: flow cytometry, fluorescence microscopy

Key Product Details

Description:	Nucleic acid binding dye. Cell permeable. Used for: cell cycle and apoptosis determination. Application: flow cytometry, fluorescence microscopy
Chemical Name:	N,N,N',N'-Tetramethyl-3,6-acridinediamine hydrochloride
Purity:	≥98% (HPLC)

View Full Technical Details for Acridine Orange hydrochloride

Technical Data
Citations

Biological Activity for Acridine Orange hydrochloride

Key information: Acridine Orange hydrochloride is a nucleic acid binding dye. Cell and organelle permeable.

Used for: cell cycle and apoptosis determination, for visual detection of nucleic acids on agarose and polyacrylamide gels, for autophagy analyze.

Application: suitable for flow cytometry and fluorescence microscopy.

Properties and Photophysical Data: emits green fluorescence when incorporated into double-stranded DNA, red fluorescence when bound electrostatically to phosphate groups of RNA or single-stranded DNA, and orange fluorescence in acidic conditions. Excitation and emission maxima (λ) are 502 nm and 525 nm, respectively (green, double strands)/ 460 nm and 650 nm, respectively (red, single strands)/ and 475 nm and 590 nm, respectively (orange, acidic conditions).

This product is supplied as a hydrochloride salt, the stoichiometry of which is batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.

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Technical Data for Acridine Orange hydrochloride

Formula	C₁₇H₁₉N₃.HCl
Storage	Store at RT
Purity	≥98% (HPLC)
PubChem ID	517204
InChi Key	VSTHNGLPHBTRMB-UHFFFAOYSA-N
Smiles	CN(C)C1=CC=C2C(N=C(C=C(N(C)C)C=C3)C3=C2)=C1.Cl
M. Wt	265.36 (Free Base)

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Acridine Orange hydrochloride

Solubility

Soluble to 10mg/ml in water

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References for Acridine Orange hydrochloride

References are publications that support the biological activity of the product.

Sato Flow cytometric analysis of Xenopus laevis and X. tropicalis blood cells using acridine orange. Sci.Rep. 2018 PMID: 30390005
McMaster Analysis of single and double stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc.Natl.Acad.Sci.USA 1977 PMID: 73185
Ratan Rapid communication: oxidative stress induces apoptosis in embryonic cortical neurons. J.Neurobiol. 2008 PMID: 7903353
Kiyoshima Chemoresistance to concanamycin A1 in human oral squamous cell carcinoma is attenuated by an HDAC inhibitor partly via suppression of Bcl-2 expression. PLoS ONE 2013 PMID: 24278362