ALDH Detection Reagent - BAAA-DA
Tocris Bioscience, part of Bio-Techne | Catalog # 7556
Key Product Details
| Description: | Fluorescent ALDH substrate and detection reagent. Used for: identification of cells with high ALDH activity. Application: flow cytometry, fluorescent-activated cell sorting (FACS) |
| Chemical Name: | (T-4)-[N-(2,2-Diethoxyethyl)-5-[(3,5-dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-1H-pyrrole-2-propanamidato-κN1]difluoroboron |
| Purity: | ≥95% (HPLC) |
| Molecular Weight: | 407.27 |
Biological Activity for ALDH Detection Reagent - BAAA-DA
Key information: ALDH Detection Reagent - BAAA-DA is a fluorescent substrate of aldehyde dehydrogenase (ALDH).
Used for: can be used in flow cytometry to sort cancer stem cells with high ALDH levels, and to isolate human hematopoietic progenitor cells. Can be used with efflux inhibitors such as Verapamil (Cat. No. 0654), Probenecid (Cat. No. 4107) or 2-deoxy-D-glucose (Cat. No. 4515) to block multi-drug resistance efflux.
Application: flow cytometry, fluorescent-activated cell sorting (FACS).
Properties and Photophysical Data: BAAA-DA is converted under acidic conditions to BAAA. BAAA, cell-permeant, is converted by intracellular ALDH into carboxylate ion amino acetate (BAA) and used to identify cells with high ALDH activity. Excitation and emission maxima (λ) are 505 nm and 512 nm, respectively; extinction coefficient = 74,595 M-1cm-1.
Optical Data for ALDH Detection Reagent - BAAA-DA
| λabs | 505 nm |
|---|---|
| λem | 512 nm |
| Extinction Coefficient (ε) | 74595 M-1cm-1 |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer