BDY 650-X, SE
Tocris Bioscience, part of Bio-Techne | Catalog # 5469
Key Product Details
| Description: | Far-red BDY (BODIPY® or boron-dipyrromethene) fluorescent dye for the labeling of amines; supplied as NHS ester |
| Chemical Name: | (T-4)-[2-[4-[2-[2-[([2,2'-Bi-1H-pyrrol]-5-yl-κN1)methylene]-2H-pyrrol-5-yl-κN]ethenyl]phenoxy]-N-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]acetamidato]difluoroboron |
| Purity: | ≥90% (HPLC) |
| Molecular Weight: | 643.44 |
Biological Activity for BDY 650-X, SE
BDY 650-X, SE is a fluorescent far-red BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a seven-atom aminohexanoyl ("X") spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between the fluorophore and conjugated biomolecule. The hydrophobic nature of BDY 650-X makes it ideal for labeling lipids and cell membranes. BDY 650-X also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~646 nM; emission maximum ~660 nM.
For more information on how BDY 650-X, SE may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.
BODIPY® is a registered trademark of Molecular Probes.
Optical Data for BDY 650-X, SE
| Emission Color | Far Red |
|---|---|
| Brightness | |
| λabs | 646 nm |
| λem | 660 nm |
| Extinction Coefficient (ε) | 102000 M-1cm-1 |
| Quantum Yield (φ) | 0.52 |
| Closest Laser line | 640 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.04 |
| Correction Factor 280 | 0.04 |
| Application | Confocal fluorescence, fluorescence polarization assays and two-photon excitation (TPE) microscopy |
| Alternative For | BDY 630-X, SE; Cy5; Alexa Fluor 647 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
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