BDY FL VH032
Tocris Bioscience, part of Bio-Techne | Catalog # 7483
Key Product Details
| Description: | High-affinity VHL fluorescent probe for TR-FRET and FP assays |
| Chemical Name: | (2S,4R)-1-((S)-2-(tert-Butyl)-21-(5,5-difluoro-7,9-dimethyl-5H-5λ4,6λ4-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-3-yl)-4,19-dioxo-6,9,12,15-tetraoxa-3,18-diazahenicosanoyl)-4-hydroxy-N-(4-(4-methylthiazol-5-yl)benzyl)pyrrolidine-2-carboxamide |
| Purity: | ≥95% (HPLC) |
| Molecular Weight: | 937.91 |
Biological Activity for BDY FL VH032
BDY FL VH032 is a high-affinity VHL fluorescent probe (Kd = 3.01 nM) with application in time-resolved fluorescence resonance energy-transfer (TR-FRET) assays for high-throughput identification and characterization of VHL ligands. BDY FL VH032 consists of a derivative of von Hippel-Lindau (VHL) ligand VH 032 (Cat. No. 6911) and a fluorophore BDY FL (Cat. No. 5465) (with excitation peak at 504 nm and emission peak at 520 nm), joined by a polyethylene glycol (PEG) 4 linker. The BDY FL VH032 VHL TR-FRET signal is stable through the 90 - 300 min incubation time, and the TR-FRET binding assay is sensitive, selective and resistant to assay interference. BDY FL VH032 also exhibits high VHL affinity in a fluorescence polarization (FP) assay. Excitation maximum ~ 504 nm; emission maximum ~ 520 nm.
Licensing Information
Sold under license from St. Jude Children's Research Hospital
Optical Data for BDY FL VH032
| Emission Color | Green |
|---|---|
| Brightness | |
| λabs | 502 nm |
| λem | 510 nm |
| Closest Laser line | 488 nm |
| Application | TR-FRET and FP Assays |
| Alternative For | FAM-DEALAHypYIPMDDDFQLRSF, FAM-DEALA-Hyp-YIPD |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer