Biotinyl Tyramide

Name: Biotinyl Tyramide
Brand: Tocris Bioscience
SKU: 6241
Rating: 5 (2 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 6241

(2)

Citations (6)

COA / SDS

Reagent widely used for signal amplification in IHC and FISH

Key Product Details

Description:	Reagent widely used for signal amplification in IHC and FISH
Chemical Name:	(3aS,4S,6aR)-Hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide
Purity:	≥98% (HPLC)
Molecular Weight:	363.47

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Technical Data
Citations / Reviews (2)

Biological Activity for Biotinyl Tyramide

Biotinyl Tyramide is a reagent widely used for signal amplification in IHC and fluorescent in situ hybridization (ISH) and APEX proximity labeling. HRP catalyzes localized deposition of multiple biotinyl tyramide molecules (catalyzed reporter deposition, CARD), which can be detected using a labeled streptavidin conjugate. The localization of deposition around HRP leads to good resolution. Suitable for either fluorescence or chromogenic detection. Biotinyl Tyramide is used as a substrate for APEX proximity labeling.

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Technical Data for Biotinyl Tyramide

M. Wt	363.47
Formula	C₁₈H₂₅N₃O₃S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	41994-02-9
PubChem ID	21826423
InChi Key	VZWXNOBHWODXCW-ZOBUZTSGSA-N
Smiles	[H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCC3=CC=C(C=C3)O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Biotinyl Tyramide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	36.35	100

Preparing Stock Solutions for Biotinyl Tyramide

The following data is based on the product molecular weight 363.47.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.75 mL	13.76 mL	27.51 mL
5 mM	0.55 mL	2.75 mL	5.50 mL
10 mM	0.28 mL	1.38 mL	2.75 mL
50 mM	0.06 mL	0.28 mL	0.55 mL

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References for Biotinyl Tyramide

References are publications that support the biological activity of the product.

McKay Amplification of fluorescent in situ hybridisation signals in formalin fixed paraffin wax embedded sections of colon tumour using biotinylated tyramide. Mol.Pathol. 1997 PMID: 9536283
Bobrow Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J.Immunol.Methods 1989 PMID: 2558138