CELT-327

CELT-327 is a potent and selective fluorescent hA_2B/A₃ adenosine receptor antagonist (K_i values are 35.6 nM and 45.7 nM at A_2B and A₃ receptors respectively). It shows dose-dependent membrane fluorescent labeling in HCT116 cells expressing A_2B adenosine receptors, with rapid diffusion and homogeneous distribution of the fluorescence signal within the cell monolayer. In live HCT116 compact spheroids, CELT-327 displays homogeneous staining across the entire spheroid diameter, reaching deep spheroid regions. CELT-327 can be used as a fluorescent adenosine receptor probe for fluorescence binding assays, live cell imaging, flow cytometry, and fluorescence polarization assays. It is a suitable replacement for GPCR radiolabeling ligands. Excitation and emission maxima (λ) are 589 nm and 616 nm, respectively. Wavelengths are compatible with use as an acceptor dye in TR-FRET assays, together with the CoraFluor^™ 1 (Cat. No. 7920) TR-FRET donor.

Add 75 μL of assay buffer containing 1% of DMSO to obtain a 100 μM stock solution, and use 250 nM as a working concentration.

Sold under license from Celtarys Research