Cyanine 3 Tyramide

Name: Cyanine 3 Tyramide
Brand: Tocris Bioscience
SKU: 6457

Tocris Bioscience, part of Bio-Techne | Catalog # 6457

Citations (1)

COA / SDS

Orange fluorescent reagent widely used for signal amplification in IHC and FISH

Key Product Details

Description:	Orange fluorescent reagent widely used for signal amplification in IHC and FISH
Chemical Name:	2-[3-[(1-Ethyl-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[[2-(4-hydroxyphenyl)ethyl]amino-6-oxohexyl]-3,3-dimethyl-5-sulfo-3H-indolium inner salt
Purity:	≥95% (HPLC)
Molecular Weight:	749.94

View Full Technical Details for Cyanine 3 Tyramide

Technical Data
Citations

Biological Activity for Cyanine 3 Tyramide

Cyanine 3 Tyramide is an orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.

Optical Data for Cyanine 3 Tyramide

Emission Color	Orange
λ_abs	550 nm
λ_em	563 nm
Extinction Coefficient (ε)	150000 M^-1cm^-1
Quantum Yield (φ)	0.31
Closest Laser line	532 nm
Application	Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for Cyanine 3 Tyramide

M. Wt	749.94
Formula	C₃₉H₄₇N₃O₈S₂
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	174961-75-2
InChi Key	JPXIOSIRIWBDIC-UHFFFAOYSA-N
Smiles	CCN1C2=C(C=C(C=C2)S(O)(=O)=O)C(/C1=C\C=C\C3=[N+](CCCCCC(NCCC4=CC=C(O)C=C4)=O)C5=C(C=C(C=C5)S([O-])(=O)=O)C3(C)C)(C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Cyanine 3 Tyramide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	12	16

Preparing Stock Solutions for Cyanine 3 Tyramide

The following data is based on the product molecular weight 749.94.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.16 mM	8.33 mL	41.67 mL	83.34 mL
0.8 mM	1.67 mL	8.33 mL	16.67 mL
1.6 mM	0.83 mL	4.17 mL	8.33 mL
8 mM	0.17 mL	0.83 mL	1.67 mL

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References for Cyanine 3 Tyramide

References are publications that support the biological activity of the product.

Zhao Characterization of GABAergic neurons in the mouse lateral septum: a double fluorescence in situ hybridization and immunohistochemical study using tyramide signal amplification. PLoS One 2013 PMID: 23967349
Schriml Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size. Biotechniques 1999 PMID: 10489619
van Gijlswijk Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J.Histochem.Cytochem. 1997 PMID: 9071319
Hopman Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for in situ hybridization using CARD amplification. J.Histochem.Cytochem. 1998 PMID: 9603790
Schmidt Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide. J.Histochem.Cytochem. 1997 PMID: 9071318