Cyanine 5, SE
Tocris Bioscience, part of Bio-Techne | Catalog # 5436
Key Product Details
| Description: | Red fluorescent dye (Reactive group: NHS ester). Application: confocal microscopy, fluorescence microscopy, flow cytometry |
| Alternative Names: | Cy5 NHS Ester |
| Chemical Name: | 2-[5-[1-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium, inner salt |
| Purity: | ≥95% (HPLC) |
| Molecular Weight: | 753.88 |
Biological Activity for Cyanine 5, SE
Key information: Cyanine 5, SE is a widely used red fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines.
Application: Suitable for fluorescence microscopy, confocal microscopy and flow cytometry.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 649 nm and 666 nm, respectively; quantum yield = 0.2; extinction coefficient = 250,000 M-1cm-1; A280 correction factor = 0.04.
For more information on how Cyanine 5, SE may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.
Optical Data for Cyanine 5, SE
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 649 nm |
| λem | 666 nm |
| Extinction Coefficient (ε) | 250000 M-1cm-1 |
| Quantum Yield (φ) | 0.2 |
| Closest Laser line | 647 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.03 |
| Correction Factor 280 | 0.04 |
| Application | Fluorescence Microscopy, Confocal Microscopy, Flow cytometry |
| Alternative For | Alexa Fluor 647; DyLight 649 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
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