CyTRAK Orange (TM)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81127
Key Product Details
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Microscopy
Product Summary for CyTRAK Orange (TM)
Key Features of CyTRAK Orange (TM):
Orange fluorescent dye that preferentially stains the nucleus, but also defines the cytoplasmic area in both live and fixed preparations
Rapid staining and easy to use, without a wash or lyse step
This dual compartment label provides quick, single cell feature discrimination such as cell location, cell perimeter, cell shape and cell spread parameters
CyTRAK Orange (TM) co-excites at 488 nm with eGFP and FITC labels avoiding the need for laser alignment in confocal instruments when using this combination of probes. Since CyTRAK Orange (TM) is not excited at 633 or 647 nm, a separate series of parameters can be detected with red-exciting fluorophores such as APC, APC-Cy7.
Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8C. DO NOT FREEZE. CyTRAK Orange (TM) can come out of solution when frozen and it is difficult (but not impossible) to get it back into solution
Orange fluorescent dye that preferentially stains the nucleus, but also defines the cytoplasmic area in both live and fixed preparations
Rapid staining and easy to use, without a wash or lyse step
This dual compartment label provides quick, single cell feature discrimination such as cell location, cell perimeter, cell shape and cell spread parameters
CyTRAK Orange (TM) co-excites at 488 nm with eGFP and FITC labels avoiding the need for laser alignment in confocal instruments when using this combination of probes. Since CyTRAK Orange (TM) is not excited at 633 or 647 nm, a separate series of parameters can be detected with red-exciting fluorophores such as APC, APC-Cy7.
Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8C. DO NOT FREEZE. CyTRAK Orange (TM) can come out of solution when frozen and it is difficult (but not impossible) to get it back into solution
Product Specifications
Applications
Flow Cytometry (1:500 - 1:1000)
Fluorescence Imaging (1:1000)
Immunocytochemistry/ Immunofluorescence (1:1000)
Live Imaging Microscopy (1:1000)
Fluorescence Imaging (1:1000)
Immunocytochemistry/ Immunofluorescence (1:1000)
Live Imaging Microscopy (1:1000)
Application Notes
CyTRAK Orange (TM) is supplied as a cranberry red aqueous solution at a concentration of 5mM: the 50 ul size covers 250 Imaging assays and 100 Flow Cytometry assays. The 200 ul size covers 1,000 Imaging assays, 6,600 HCS assays, and 400 Flow Cytometry assays.
Scientific Data Images for CyTRAK Orange (TM)
Live Imaging Microscopy: CyTRAK Orange (TM) [NBP2-81127]
Live Imaging Microscopy: CyTRAK Orange (TM) [NBP2-81127] - CyTRAK Orange was diluted in EMEM culture media and applied to live HeLa cells at 5 uM (1:1000) for 30 minutes at room temperature and protected from light. Imaging was done immediately after staining without washing the cells.Flow Cytometry: CyTRAK Orange (TM) [NBP2-81127]
Flow Cytometry: CyTRAK Orange (TM) [NBP2-81127] - Nucleated cell gating by CyTRAK Orange (TM )intensity of intact, unlysed bone marrow with retained forward and side scatter characteristics.
CyTRAK Orange (TM) [NBP2-81127] - Excitation and Emission Spectra for CyTRAK Orange (TM)
Formulation, Preparation, and Storage
Purification
>97%
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C in the dark. Do not freeze.
Background: CyTRAK Orange (TM)
The excitation wavelength maximum of CyTRAK Orange (TM) is 510 nm and its emission wavelength maximum is 610 nm. CyTRAK Orange (TM) is not excited by the UV or red laser.
Additional CyTRAK Orange (TM) Products
Product Documents for CyTRAK Orange (TM)
Product Specific Notices for CyTRAK Orange (TM)
CyTRAK Orange (TM) is a registered trademark of BioStatus Limited.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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