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CyTRAK Orange (TM)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81127

Novus Biologicals, part of Bio-Techne
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NBP2-81127-200ul
NBP2-81127-50ul

Key Product Details

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Microscopy

Product Summary for CyTRAK Orange (TM)

Key Features of CyTRAK Orange (TM):
Orange fluorescent dye that preferentially stains the nucleus, but also defines the cytoplasmic area in both live and fixed preparations
Rapid staining and easy to use, without a wash or lyse step
This dual compartment label provides quick, single cell feature discrimination such as cell location, cell perimeter, cell shape and cell spread parameters
CyTRAK Orange (TM) co-excites at 488 nm with eGFP and FITC labels avoiding the need for laser alignment in confocal instruments when using this combination of probes. Since CyTRAK Orange (TM) is not excited at 633 or 647 nm, a separate series of parameters can be detected with red-exciting fluorophores such as APC, APC-Cy7.

Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8C. DO NOT FREEZE. CyTRAK Orange (TM) can come out of solution when frozen and it is difficult (but not impossible) to get it back into solution

Product Specifications

Applications

Flow Cytometry (1:500 - 1:1000)
Fluorescence Imaging (1:1000)
Immunocytochemistry/ Immunofluorescence (1:1000)
Live Imaging Microscopy (1:1000)

Application Notes

CyTRAK Orange (TM) is supplied as a cranberry red aqueous solution at a concentration of 5mM: the 50 ul size covers 250 Imaging assays and 100 Flow Cytometry assays. The 200 ul size covers 1,000 Imaging assays, 6,600 HCS assays, and 400 Flow Cytometry assays.

Scientific Data Images for CyTRAK Orange (TM)

Live Imaging Microscopy: CyTRAK Orange (TM) [NBP2-81127]

Live Imaging Microscopy: CyTRAK Orange (TM) [NBP2-81127]

Live Imaging Microscopy: CyTRAK Orange (TM) [NBP2-81127] - CyTRAK Orange was diluted in EMEM culture media and applied to live HeLa cells at 5 uM (1:1000) for 30 minutes at room temperature and protected from light. Imaging was done immediately after staining without washing the cells.
Flow Cytometry: CyTRAK Orange (TM) [NBP2-81127]

Flow Cytometry: CyTRAK Orange (TM) [NBP2-81127]

Flow Cytometry: CyTRAK Orange (TM) [NBP2-81127] - Nucleated cell gating by CyTRAK Orange (TM )intensity of intact, unlysed bone marrow with retained forward and side scatter characteristics.
CyTRAK Orange (TM) [NBP2-81127] - Excitation and Emission Spectra for CyTRAK Orange (TM)

Formulation, Preparation, and Storage

Purification

>97%

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C in the dark. Do not freeze.

Background: CyTRAK Orange (TM)

CyTRAK Orange (TM) is an orange fluorescent dye that stains the nucleus and cytoplasm but with different intensities. It functions as a DNA intercalating probe and does not crosslink with or esterify cytoplasmic proteins. Since nuclear staining is stronger than cytoplasmic staining, both cellular compartments can be easily identified during high content imaging screening. In addition CyTRAK Orange (TM) preferentially stains arrested / senescent cells compared to healthy cells, thus enabling easy identification and separation of these two distinct cellular populations.

The excitation wavelength maximum of CyTRAK Orange (TM) is 510 nm and its emission wavelength maximum is 610 nm. CyTRAK Orange (TM) is not excited by the UV or red laser.

Additional CyTRAK Orange (TM) Products

Product Documents for CyTRAK Orange (TM)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CyTRAK Orange (TM)

CyTRAK Orange (TM) is a registered trademark of BioStatus Limited.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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