DFHO

Name: DFHO
Brand: Tocris Bioscience
SKU: 6434

Tocris Bioscience, part of Bio-Techne | Catalog # 6434

Citations (4)

COA / SDS

RFP fluorophore mimic for imaging RNA in living cells; activated by binding Corn aptamers

Key Product Details

Description:	RFP fluorophore mimic for imaging RNA in living cells; activated by binding Corn aptamers
Chemical Name:	4-(3,5-Difluoro-4-hydroxybenzylidene)-1-methyl-5-oxo-4,5-dihydro-1H-imidazole-2-carbaldehyde oxime
Purity:	≥98% (HPLC)
Molecular Weight:	281.22

View Full Technical Details for DFHO

Technical Data
Citations

Biological Activity for DFHO

DFHO is a mimic of red fluorescent protein (RFP) fluorophore for imaging RNA in living cells. DFHO fluorescence is activated by binding to Corn aptamer (K_d = 70 nM) or Squash aptamer (K_d = 54 nM). Excitation/emission maxima (λ) = 505/545 nm; extinction coefficient = 29000 M^-1 cm^-1.

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Technical Data for DFHO

M. Wt	281.22
Formula	C₁₂H₉F₂N₃O₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1420815-34-4
Smiles	CN1C(/C=N/O)=N/C(C1=O)=C\C2=CC(F)=C(C(F)=C2)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DFHO

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	28.12	100

Preparing Stock Solutions for DFHO

The following data is based on the product molecular weight 281.22.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.56 mL	17.78 mL	35.56 mL
5 mM	0.71 mL	3.56 mL	7.11 mL
10 mM	0.36 mL	1.78 mL	3.56 mL
50 mM	0.07 mL	0.36 mL	0.71 mL

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References for DFHO

References are publications that support the biological activity of the product.

Truong The fluorescent aptamer Squash extensively repurposes the adenine riboswitch fold. Nat.Chem.Biol. 2022 PMID: 34937911
Warner A homodimer interface without base pairs in an RNA mimic of red fluorescent protein. Nat.Chem.Biol. 2017 PMID: 28945234
Dey Repurposing an adenine riboswitch into a fluorogenic imaging and sensing tag. Nat.Chem.Biol. 2022 PMID: 34937909
Song Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex. Nat.Chem.Biol. 2017 PMID: 28945233