Di 4 ANEPPS

Name: Di 4 ANEPPS
Brand: Tocris Bioscience
SKU: 7324

Tocris Bioscience, part of Bio-Techne | Catalog # 7324

Citations (1)

COA / SDS

Voltage-sensitive probe; used to detect changes in membrane potential in electrophysiology protocols

Key Product Details

Description:	Voltage-sensitive probe; used to detect changes in membrane potential in electrophysiology protocols
Chemical Name:	6-[2-(N,N-Dibutylamino)naphthyl]ethenyl-4'-pyridinium propanesulfonate
Purity:	≥90% (HPLC)
Molecular Weight:	480.67

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Technical Data
Citations

Biological Activity for Di 4 ANEPPS

Di 4 ANEPPS is a voltage-sensitive dye, also known as an electrochromic probe, which fluoresces in response to change in electrical potential across a cellular membrane. Di 4 ANEPPS fluoresces only when membrane bound; change in electrical potential causes an intramolecular charge transfer and blue-shift in the excitation spectra. Di 4 ANEPPS is commonly used to detect transient changes in membrane potential in excitable cells such as neurons and cardiac cells. Excitation ratio measurements can be used to determine membrane potential.

In model phospholipid membranes Ex/Em maxima are 465/635 nm, however spectral properties are highly dependent on membrane environment.

It is recommended to prepare stock solutions in DMSO.

Optical Data for Di 4 ANEPPS

Emission Color	Red
λ_abs	465 nm
λ_em	635 nm
Application	Electrophysiology, Fluorescence Microscopy

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for Di 4 ANEPPS

M. Wt	480.67
Formula	C₂₈H₃₆N₂O₃S
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	90134-00-2
PubChem ID	6438327
InChi Key	HAPJROQJVSPKCJ-UHFFFAOYSA-N
Smiles	[O-]S(CCC[N+](C=C1)=CC=C1/C=C/C(C=C2C=C3)=CC=C2C=C3N(CCCC)CCCC)(=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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References for Di 4 ANEPPS

References are publications that support the biological activity of the product.

Tominga & Tominga A new nonscanning confocal microscopy module for functional voltage-sensitive dye and Ca²⁺ imaging of neuronal circuit activity. J.Neurophysiol. 2013 PMID: 23615547
Asamoah A fluorometric approach to local electric field measurements in a voltage-gated ion channel. Neuron. 2003 PMID: 12526775
Loew A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations. J.Membr.Biol. 1992 PMID: 1469705