DRAQ7 (TM)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81126
Key Product Details
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Microscopy
Product Summary for DRAQ7 (TM)
Key Features of DRAQ7 (TM) :
Far-red viability dye used for investigating dead or membrane compromised cells, notably for dead cell exclusion in flow cytometry, single-cell RNAseq and GWAS
Does not enter intact, live cells and acts as an ideal replacement for propidium iodide (PI) and 7-AAD
Serves as a component of apoptosis assays, for any fluorescence-based cell analysis platform, and in cell health / in vitro toxicity assays
Rapid staining and easy to use, without the need for a wash step
Non-toxic and optimal for long-term imaging studies, up to several days
Minimal photobleaching
Spectrally compatible with GFP and FITC labels.
DRAQ7 (TM) is supplied as a blue aqueous solution and shipped at ambient temperature, but on receipt packs should be stored at 2-8C. DO NOT FREEZE.
Far-red viability dye used for investigating dead or membrane compromised cells, notably for dead cell exclusion in flow cytometry, single-cell RNAseq and GWAS
Does not enter intact, live cells and acts as an ideal replacement for propidium iodide (PI) and 7-AAD
Serves as a component of apoptosis assays, for any fluorescence-based cell analysis platform, and in cell health / in vitro toxicity assays
Rapid staining and easy to use, without the need for a wash step
Non-toxic and optimal for long-term imaging studies, up to several days
Minimal photobleaching
Spectrally compatible with GFP and FITC labels.
DRAQ7 (TM) is supplied as a blue aqueous solution and shipped at ambient temperature, but on receipt packs should be stored at 2-8C. DO NOT FREEZE.
Product Specifications
Applications
Flow Cytometry (1:100)
Fluorescence Imaging (1:60 - 1:100)
Immunocytochemistry/ Immunofluorescence (1:60)
Live Imaging Microscopy (1:100)
Fluorescence Imaging (1:60 - 1:100)
Immunocytochemistry/ Immunofluorescence (1:60)
Live Imaging Microscopy (1:100)
Application Notes
DRAQ7 (TM) is supplied at a concentration of 0.3mM: the 250 ul size allows for 50 Flow Cytometry assays and 250 Cell Health assays whereas the 1ml size allows for 200 Flow Cytometry assays and 1,000 Cell Health assays. DRAQ7 (TM) can be diluted in culture media (e.g. RPMI 1640) and physiological buffers (eg PBS, Hankss, etc.) and mixed with fixatives such as formaldehyde. DRAQ7 (TM) has many applications in imaging, cytometry and screening and is highly compatible with existing protocols across a wide range of instrumentation platforms.
Scientific Data Images for DRAQ7 (TM)
![Flow Cytometry: DRAQ7 (TM) [NBP2-81126]](https://resources.bio-techne.com/images/products/DRAQ7-TM-Flow-Cytometry-NBP2-81126-img0003.jpg "Flow Cytometry: DRAQ7 (TM) [NBP2-81126]")
Flow Cytometry: DRAQ7 (TM) [NBP2-81126]
Flow Cytometry: DRAQ7 (TM) [NBP2-81126] - Lymphoma cells treated with increasing quantities of staurosporine (STS). reports STS-induced apoptosis and cell death in dose-dependent manner with clear separation of positive and negative events.![Live Imaging Microscopy: DRAQ7 (TM) [NBP2-81126]](https://resources.bio-techne.com/images/products/DRAQ7-TM-Live-Imaging-Microscopy-NBP2-81126-img0005.jpg "Live Imaging Microscopy: DRAQ7 (TM) [NBP2-81126]")
Live Imaging Microscopy: DRAQ7 (TM) [NBP2-81126]
Live Imaging Microscopy: DRAQ7 (TM) [NBP2-81126] - DRAQ7(TM) was added directly to unfixed THP-1 cells in RPMI + 20% FBS culture media at 3 uM (1:100) for 30 minutes at room temperature and protected from light. Imaging was done immediately after staining without washing the cells.![Immunocytochemistry/ Immunofluorescence: DRAQ7 (TM) [NBP2-81126]](https://resources.bio-techne.com/images/products/DRAQ7-TM-Immunocytochemistry-Immunofluorescence-NBP2-81126-img0004.jpg "Immunocytochemistry/ Immunofluorescence: DRAQ7 (TM) [NBP2-81126]")
Immunocytochemistry/ Immunofluorescence: DRAQ7 (TM) [NBP2-81126]
Immunocytochemistry/Immunofluorescence: DRAQ7 (TM) [NBP2-81126] - Formaldehyde-fixed U2OS cells labelled with (red, nuclei+D8 and AlexaFluor 488 antibody to beta-tubulin (green).
Formulation, Preparation, and Storage
Purification
>97%
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C in the dark. Do not freeze.
Background: DRAQ7 (TM)
The excitation wavelength maxima of DRAQ7 (TM) are 600 nm / 646 nm but it can also be suboptimally excited by the 488nm (blue) laser for flow cytometry. The emission wavelength maximum of this viability dye is 697 nm when bound to dsDNA. The fluorescence signal is proportional and stoichiometric to the amount of bound DNA.
DRAQ7 (TM) is a registered trademark of BioStatus Limited.
Additional DRAQ7 (TM) Products
Product Documents for DRAQ7 (TM)
Product Specific Notices for DRAQ7 (TM)
DRAQ7 (TM) is a registered trademark of BioStatus Limited.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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