FAM-DEALA-Hyp-YIPD

Name: FAM-DEALA-Hyp-YIPD
Brand: Tocris Bioscience
SKU: 7287

Tocris Bioscience, part of Bio-Techne | Catalog # 7287

COA / SDS

Fluorescent HIF-1α peptide; can be used to evaluate the effect of VHL binding on degradation activity

Key Product Details

Description:	Fluorescent HIF-1α peptide; can be used to evaluate the effect of VHL binding on degradation activity
Purity:	≥95% (HPLC)
Molecular Weight:	1477.48

View Full Technical Details for FAM-DEALA-Hyp-YIPD

Technical Data
Citations

Biological Activity for FAM-DEALA-Hyp-YIPD

FAM-DEALA-Hyp-YIPD is a fluorescent HIF-1α peptide (K_d = 180-560 nM). Can be used to assess VHL binding in Fluorescence Polarization (FP) displacement assay, and evaluate the effect of VHL binding on degradation activity. Excitation maximum = 485 nm, emission maximum = 535 nm. Longer peptide version, FAM-DEALAHypYIPMDDDFQLRSF (Cat. No. 7452), also available.

Optical Data for FAM-DEALA-Hyp-YIPD

Emission Color	Green
λ_abs	485 nm
λ_em	520 nm
Closest Laser line	488 nm
Application	Fluorescence Polarization (FP) Displacement Assays

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for FAM-DEALA-Hyp-YIPD

M. Wt	1477.48
Formula	C₇₁H₈₄N₁₀O₂₅
Single Letter Sequence	DEALAXYIPD (Modifications: Asp-1 = 5-FAM-Asp, X-6 = Hyp)
Storage	Store at -20°C
Purity	≥95% (HPLC)
InChi Key	CWNCTVVEODBMBK-RHNKRQRVSA-N
Smiles	O=C([C@@H]1CCCN1C([C@H]([C@@H](CC)C)NC([C@H](CC2=CC=C(O)C=C2)NC([C@@H]3C[C@@H](O)CN3C([C@@H](NC([C@H](CC(C)C)NC([C@@H](NC([C@H](CCC(O)=O)NC([C@@H](NC(C4=CC(C(O5)=O)=C(C=C4)C65C7=CC=C(O)C=C7OC8=C6C=CC(O)=C8)=O)CC(O)=O)=O)=O)C)=O)=O)C)=O)=O)=O)=O)N[C@@H](CC(O)=O)C(O)=O.O=C([C@@H]9CCCN9C([C@H]([C@@H](CC)C)NC([C@H](CC%10=CC=C(O)C=C%10)NC([C@@H]%11C[C@@H](O)CN%11C([C@@H](NC([C@H](CC(C)C)NC([C@@H](NC([C@H](CCC(O)=O)NC([C@@H](NC(C(C=C%12)=CC(C%13%14C%15=CC=C(O)C=C%15OC%16=C%13C=CC(O)=C%16)=C%12C(O%14)=O)=O)CC(O)=O)=O)=O)C)=O)=O)C)=O)=O)=O)=O)N[C@@H](CC(O)=O)C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for FAM-DEALA-Hyp-YIPD

Solubility

Soluble to 1mg/ml in 0.01M PBS

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References for FAM-DEALA-Hyp-YIPD

References are publications that support the biological activity of the product.

Ahn HIF-1α peptide derivatives with modifications at the hydroxyproline residue as activators of HIF-1α. Bioorg.Med.Chem.Lett. 2009 PMID: 19515556
Crews Identification and characterization of von Hippel-Lindau-recruiting Proteolysis Targeting Chimeras (PROTACs) of TANK-binding kinase 1. J.Med.Chem. 2018 PMID: 28692295
Lucas Surface probing by fragment-based screening and computational methods identifies ligandable pockets on the von Hippel-Lindau (VHL) E3 ubiquitin ligase. J.Med.Chem. 2018 PMID: 30040896
Roy SPR-measured kinetics of PROTAC ternary complexes influence target degradation rate. ACS Chem.Biol. 2019 PMID: 30721025
Buckley Targeting the von Hippel-Lindau E3 ubiquitin ligase using small molecules to disrupt the VHL/HIF-1a interaction. J.Am.Chem.Soc. 2012 PMID: 22369643