Flutax 1
Tocris Bioscience, part of Bio-Techne | Catalog # 2226
Key Product Details
| Description: | Green-fluorescent taxol derivative; binds microtubules. Used for: imaging microtubules in live cells. Application: Fluorescent microscopy |
| Chemical Name: | (2aR,4S,4aS,6R,9S,11S,12S,12aR,12bS)-6,12b-Bis(acetyloxy)-9-[(2R,3S)-3-(benzoylamino)-2-hydroxy-1-oxo-3-phenylpropoxy]-12-(benzoyloxy)-2a,3,4,4a,5,6,9,10,11,12,12a,12b-dodecahydro-11-hydroxy-4a,8,13,13-tetramethyl-5-oxo-7,11-methano-1H-cyclodeca[3,4]benz[1,2-b]oxet-4-yl ester N-[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]-L-alanine |
| Purity: | ≥95% (HPLC) |
| Molecular Weight: | 1283.3 |
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Flutax 1 labeled HeLa cells. Flutax 1 was diluted in HBSS and applied to live HeLa cells at a concentration of 2 μM. HeLa cells were incubated for 1 hour at 37°C. After incubation was complete, Flutax 1 was washed off, and fresh HBSS was applied to the cells. Cells were not fixed because Flutax 1 staining is not retained at all after fixation (photobleach). Staining can be done only on Live cells. Flutax 1 staining in live cells diminishes very rapidly when exposed to light, so the cells should be exposed to light as little as possible to retain good fluorescent signal. |
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