FURA-2AM

Name: FURA-2AM
Brand: Tocris Bioscience
SKU: 2220
Rating: 5 (3 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 2220

(3)

Citations (7)

COA / SDS

Fluorescent Ca2+ indicator

Key Product Details

Description:	Fluorescent Ca²⁺ indicator
Chemical Name:	2-[6-[bis[2-[(Acetyloxy)methoxy]-2-oxoethyl]amino]-5-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid (acetyloxy)methyl ester
Purity:	≥95% (HPLC)
Molecular Weight:	1001.85

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Technical Data
Citations / Reviews (3)

Biological Activity for FURA-2AM

FURA-2AM is a fluorescent ratiometric Ca²⁺ indicator (K_d Ca²⁺ = 145 nM). FURA-2AM is selective for Ca²⁺ over other divalent cations Mg²⁺, Zn²⁺, Fe²⁺ and Mn²⁺. FURA-2AM binds to free intracellular calcium and is used to determine [Ca²⁺]_i concentration.

This product is typically prepared in DMSO. F 127 (Cat. No. 6253) for the solubilization of FURA-2AM is also available.

Optical Data for FURA-2AM

λ_abs	371 nm
λ_em	474 nm
Extinction Coefficient (ε)	30000 M^-1cm^-1
Cell Permeable	Yes
Application	Flow Cytometry, Fluorescent Microscopy

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Technical Data for FURA-2AM

M. Wt	1001.85
Formula	C₄₄H₄₇N₃O₂₄
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	108964-32-5
PubChem ID	90488749
InChi Key	JTDRWQMCMPIBNJ-UHFFFAOYSA-N
Smiles	CC1=CC(OCCOC2=C(N(CC(OCOC(C)=O)=O)CC(OCOC=O)=O)C=C(OC(C4=NC=C(C(OCOC(C)=O)=O)O4)=C3)C3=C2)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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References for FURA-2AM

References are publications that support the biological activity of the product.

Wang Sildenafil inhibits human pulmonary artery smooth muscle cell proliferation by decreasing capacitative Ca²⁺ entry. J.Pharmacol.Sci. 2008 PMID: 18818482
Grynkiewicz A new generation of Ca²⁺ indicators with greatly improved fluorescence properties. J.Biol.Chem. 1985 PMID: 3838314