Hoechst 33342

Name: Hoechst 33342
Brand: Tocris Bioscience
SKU: 5117
Rating: 5 (3 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 5117

COA / SDS

Blue-fluorescent dye for DNA staining. Cell permeable. Used for: nuclear counterstain, apoptosis analysis, fixed and live-cell staining. Application: flow cytometry, confocal microscopy

Key Product Details

Chemical Name:	2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride
Purity:	≥98%

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Technical Data
Citations / Reviews (4)

Biological Activity for Hoechst 33342

Key information: Hoechst 33342 is a blue-fluorescent dye for DNA staining. Cell permeable. Suitable for fixed and live-cell staining. Commonly used as a counterstain in fluorescence microscopy.

Used for: nuclear counterstain, DNA visualization, cell cycle studies, apoptosis analysis.

Application: flow cytometry, confocal microscopy.

Properties and Photophysical Data: Hoechst 33342 binds to the AT-rich regions of the minor grove in DNA which renders it specific for nuclear chromatin. Excitation and emission maxima (λ) are 350 nm and 461 nm, respectively.

Scientific Data Examples for Hoechst 33342

	Hoechst 33342 \| CAS No. 875756-97-1 Fluorescent dye for staining DNA

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Technical Data for Hoechst 33342

Formula	C₂₇H₂₈N₆O.3HCl
Storage	Store at -20°C
Purity	≥98%
CAS Number	875756-97-1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Hoechst 33342

Solubility

Soluble Soluble to 50 mM in water and to 50 mM in DMSO

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References for Hoechst 33342

References are publications that support the biological activity of the product.

Portugal and Waring Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochim.Biophys.Acta., 1988;949:158 PMID: 2449244
Loken Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter. Cytometry, 1980;1:136 PMID: 7028425