Hoechst Janelia Fluor® 526
Tocris Bioscience, part of Bio-Techne | Catalog # 7313
Key Product Details
| Description: | Fluorogenic, green-emitting DNA probe. Used for: fixed and live-cell imaging. Application: confocal microscopy, flow cytometry, super resolution microscopy (SRM) |
| Alternative Names: | JF526-HST |
| Chemical Name: | 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-((2-(2-(2-(4-(4-(6-(4-methylpiperazin-1-yl)-1H,3'H-[2,5'-bibenzo[d]imidazol]-2'-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)benzoate tetratrifluoroacetate |
| Purity: | ≥95% (HPLC) |
| Molecular Weight: | 1641.27 |
Biological Activity for Hoechst Janelia Fluor® 526
Key information: Hoechst Janelia Fluor® 526 is a fluorogenic, green-emitting DNA probe. Suitable for live cell imaging.
Used for: fixed and live-cell imaging.
Application: confocal microscopy, flow cytometry, super resolution microscopy (SRM).
Properties and Photophysical Data: preferentially stains and binds minor groove of AT-rich regions. When bound to A-T DNA, Hoechst Janelia Fluor® 526 exhibits a quantum yield (QY) of 0.126; when bound to G-C DNA Hoechst Janelia Fluor® 526 exhibits a quantum yield of 0.04. Fluorogenic, it fluoresces only once bound to DNA, enabling hassle-free no-wash experiments. Hoechst Janelia Fluor® 526 can be combined with fluorogenic red-emitting DNA probe Hoechst Janelia Fluor® 646 (Cat. No. 6804) for multiplexing experiments. Excitation and emission maxima (λ) are 531 nm and 549 nm, respectively; quantum yield = 0.126; extinction coefficient = 118,000 M-1cm-1.
To measure the absorbance spectrum of Hoechst Janelia Fluor® 526 we recommend the following solvent: TFE plus 0.1% TFA.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Hoechst Janelia Fluor® 526
| Emission Color | Yellow |
|---|---|
| λabs | 531 nm |
| λem | 549 nm |
| Extinction Coefficient (ε) | 118,000 M-1cm-1 |
| Quantum Yield (φ) | 0.126 |
| Application | Confocal Microscopy, Flow Cytometry, Super Resolution Microscopy (SRM) |
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Application of Hoechst Janelia Fluor® 526 in U2OS cells. Fixed U2OS cells were stained with Hoechst Janelia Fluor® 526 (2 µM) for 1 hour and imaged without an intermediate washing step on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 514 nm / emission: 526–615 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus. |
Plan Your Experiments
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