Indo 1AM

Name: Indo 1AM
Brand: Tocris Bioscience
SKU: 6704

Tocris Bioscience, part of Bio-Techne | Catalog # 6704

COA / SDS

Fluorescent Ca2+ indicator

Key Product Details

Description:	Fluorescent Ca²⁺ indicator
Chemical Name:	2-[4-[Bis[2-[(acetoxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylic acid (acetoxy)methyl ester
Purity:	≥90% (HPLC)
Molecular Weight:	1009.91

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Technical Data
Citations

Biological Activity for Indo 1AM

Indo 1AM is a fluorescent Ca²⁺ indicator (K_d for Ca²⁺ = 250 nM). Cell-permeable. Displays ratiometric emission wavelength change upon Ca²⁺ binding. Excitation/emission λ are 355/475 nm for no Ca2+ and 355/401 nm for high Ca²⁺. Absorption maxima are 349 and 331 nm for free anion and Ca complex, respectively. Respective emission maxima are 485 and 410 nm. Quantum yield ~0.5. Ideal for flow cytometry..

This product is typically prepared in DMSO.

Optical Data for Indo 1AM

Emission Color	Blue
λ_abs	355 nm
λ_em	474 nm
Closest Laser line	350 nm
Cell Permeable	Yes
Application	Flow Cytometry, Fluorescence Microscopy

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Technical Data for Indo 1AM

M. Wt	1009.91
Formula	C₄₇H₅₁N₃O₂₂
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	112926-02-0
PubChem ID	123918
InChi Key	CAWBRCOBJNWRLK-UHFFFAOYSA-N
Smiles	CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C1=C(OCCOC2=C(C=CC(=C2)C2=CC3=C(N2)C=C(C=C3)C(=O)OCOC(C)=O)N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C=C(C)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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References for Indo 1AM

References are publications that support the biological activity of the product.

Grynkiewicz A new generation of Ca²⁺ indicators with greatly improved fluorescence properties. J.Biol.Chem. 1985 PMID: 3838314