Janelia Fluor® 526, NHS ester
Tocris Bioscience, part of Bio-Techne | Catalog # 7316
Key Product Details
| Description: | Fluorogenic yellow dye (Reactive group: NHS ester). Application: confocal microscopy, two-color lattice light-sheet microscopy, super resolution microscopy |
| Alternative Names: | JF 526,NHS,JF526 |
| Chemical Name: | 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate |
| Molecular Weight: | 659.5 |
Biological Activity for Janelia Fluor® 526, NHS ester
Key Information: Janelia Fluor® 526, NHS ester is a fluorogenic yellow fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines.
Application: Suitable for confocal microscopy, two-color lattice light-sheet microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Cell permeable.
Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Can be multiplexed for two color imaging with Janelia Fluor® 635 NHS ester (Cat. No. 6419). Excitation and emission maxima (λ) are 526 nm and 550 nm, respectively; quantum yield = 0.87; extinction coefficient = 118,000 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 0.005.
To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.
Please see the protocol for further information on protein/antibody labeling and conjugation.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
This product has been recently renamed. The previous name for this product was Janelia Fluor® 526, SE.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 526, NHS ester
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 526 nm |
| λem | 550 nm |
| Extinction Coefficient (ε) | 118,000 M-1cm-1 |
| Quantum Yield (φ) | 0.87 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy (SRM), Two-color Lattice Light-sheet Microscopy |
| Alternative For | Alexa Fluor® 532, Alexa Fluor® 514, Atto 532, CF514, CF532 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
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