Janelia Fluor® 549, free acid
Tocris Bioscience, part of Bio-Techne | Catalog # 6503
Key Product Details
| Description: | Yellow dye (Reactive group: free acid). Application: flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging |
| Chemical Name: | 3,6-Di-1-azetidinyl-9-(2,5-dicarboxyphenyl)xanthylium, inner salt trifluoroacetate |
| Purity: | ≥90% (HPLC) |
| Molecular Weight: | 568.5 |
Biological Activity for Janelia Fluor® 549, free acid
Key Information: Janelia Fluor® 549, free acid is a yellow fluorescent dye; supplied with a free acid reactive group. Suitable for live cell imaging.
Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-tag® ligands. Cell permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 549 nm and 571 nm, respectively; quantum yield = 0.88; extinction coefficient = 101,000 M-1cm-1, A280 correction factor = 0.169; lactone-zwitterion equilibrium constant (KL-Z) = 3.5.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 549, free acid
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 549 nm |
| λem | 571 nm |
| Extinction Coefficient (ε) | 101,000 M-1cm-1 |
| Quantum Yield (φ) | 0.88 |
| Closest Laser line | 532 nm |
| Reactive Group | Free acid |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.169 |
| Application | Confocal Microscopy, dSTORM, STED, Flow Cytometry, Live-cell Imaging |
| Alternative For | Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer