Janelia Fluor® 635b, Maleimide
Tocris Bioscience, part of Bio-Techne | Catalog # 8156
Key Product Details
| Description: | Blinking fluorescent dye (Reactive group: maleimide). Application: super resolution microscopy (SRM) including SMLM |
| Chemical Name: | N-(2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)-3,7-bis(3-fluoroazetidin-1-yl)-5,5-dimethyl-3'H,5H-spiro[dibenzo[b,e]siline-10,1'-isobenzofuran]-6'-carboxamide |
| Purity: | ≥90% (HPLC) |
| Molecular Weight: | 640.76 |
Biological Activity for Janelia Fluor® 635b, Maleimide
Spontaneously Blinking Janelia Fluor® Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor® technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments.
Key Information: Janelia Fluor® 635b, Maleimide is a spontaneously blinking fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity) for the labeling of protein. Suitable to characterize the physical properties of dense biomolecule condensates and complex macromolecular assemblies.
Application: suitable for super resolution microscopy (SRM) including single-molecule localization microscopy (SMLM).
Properties and Photophysical Data: derived from Janelia Fluor® 635, Maleimide (Cat. No. 8027). Excitation and emission maxima (λ) are 651 nM and 667 nM, respectively.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 635b, Maleimide
| λabs | 651 nm |
|---|---|
| λem | 667 nm |
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Application of Janelia Fluor® 635b, Maleimide. 3D SMLM map of a FUS coacervate aged for 12 h containing 20 nM FUSA2C-JF635b; inset shows brightfield image; scale bar = 4 μm; n = 31,123 total localizations; colors indicate localization density within a 100 nm radius sphere: black = 1; orange 2–10; green = 11–20; cyan = 21–40; magenta = 41–100. Image kindly provided by Luke D. Lavis, Janelia Research Campus. For more information on this work read Luke D. Lavis et al (2024) A series of spontaneously blinking dyes for super-resolution microscopy. ACS Chem.Biol. bioRxiv. PMID: 38766149 |
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