Janelia Fluor® 646, Maleimide
Tocris Bioscience, part of Bio-Techne | Catalog # 6590
Key Product Details
| Description: | Red dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging |
| Chemical Name: | 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)benzoate |
| Purity: | ≥95% (HPLC) |
| Molecular Weight: | 618.75 |
Biological Activity for Janelia Fluor® 646, Maleimide
Key information: Janelia Fluor® 646, Maleimide is a red fluorogenic fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.
Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Can be multiplexed for two color imaging with Janelia Fluor® 549 SE (Cat. No. 6147). Cell permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor = 0.19; lactone-zwitterion equilibrium constant (KL-Z) = 0.0012.
Please see the product protocol for further information and a guide to protein/antibody labeling.
To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 646, Maleimide
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 646 nm |
| λem | 664 nm |
| Extinction Coefficient (ε) | 152,000 M-1cm-1 |
| Quantum Yield (φ) | 0.54 |
| Closest Laser line | 640 nm |
| Reactive Group | Maleimide |
| Reactivity | Thiols |
| Cell Permeable | Yes |
| Correction Factor 260 | 0.09 |
| Correction Factor 280 | 0.19 |
| Application | Confocal Microscopy, dSTORM, STED, Flow Cytometry, Live-cell Imaging |
| Alternative For | Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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