Janelia Fluor® 646b, NHS ester
Tocris Bioscience, part of Bio-Techne | Catalog # 8159
Key Product Details
| Description: | Blinking fluorescent dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM) including SMLM |
| Chemical Name: | 2,5-Dioxopyrrolidin-1-yl 3,7-di(azetidin-1-yl)-5,5-dimethyl-3'H,5H-spiro[dibenzo[b,e]siline-10,1'-isobenzofuran]-6'-carboxylate |
| Purity: | ≥90% (HPLC) |
| Molecular Weight: | 579.73 |
Biological Activity for Janelia Fluor® 646b, NHS ester
Spontaneously Blinking Janelia Fluor® Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor® technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments.
Key Information: Janelia Fluor® 646b, NHS ester is a spontaneously blinking fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for DNA labeling using amine-terminated oligonucleotides in RNA-FISH experiments.
Application: suitable for super resolution microscopy (SRM) including single-molecule localization microscopy (SMLM).
Properties and Photophysical Data: derived from Janelia Fluor® 646, SE (Cat. No. 6148). Excitation and emission maxima (λ) are 662 nM and 678nM, respectively.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 646b, NHS ester
| λabs | 679 nm |
|---|---|
| λem | 662 nm |
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Application of Janelia Fluor® 646b, NHS ester. SOFI RNA-FISH image of mouse embryo fibroblast cells expressing MS2 in the 3′ UTR of the beta-actin gene and labeled with Janelia Fluor®646b-oligonucleotide from Janelia Fluor® 646b, NHS ester; scale bar: 5 μm. Image kindly provided by Luke D. Lavis, Janelia Research Campus. For more information on this work read Luke D. Lavis et al (2024) A series of spontaneously blinking dyes for super-resolution microscopy. ACS Chem.Biol. bioRxiv. PMID: 38766149 |
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