JFX™ 650, Maleimide
Tocris Bioscience, part of Bio-Techne | Catalog # 8162
Key Product Details
| Description: | Deuterated Janelia Fluor® dye (Reactive group: Maleimide). Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging |
| Chemical Name: | 2-(5,5-Dimethyl-3-(pyrrolidin-1-ium-1-ylidene-d8)-7-(pyrrolidin-1-yl-d8)-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)benzoate trifluoroacetate |
| Purity: | ≥90% (HPLC) |
| Molecular Weight: | 776.94 |
Biological Activity for JFX™ 650, Maleimide
Key Information: JFX™ 650, Maleimide is a red fluorescent deuterated Janelia Fluor® dye; supplied with a Maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.
Application: Suitable for confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Cell permeable.
Properties and Photophysical Data: JFX™ 650, Maleimide shows enhanced brightness, photostability and chromostability. Maleimide can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 650 nm and 667 nm, respectively; quantum yield = 0.53; extinction coefficient = 17,600 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 0.015.
Please see the product protocol for further information and a guide to protein/antibody labeling.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
JFX is a trademark owned by the Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for JFX™ 650, Maleimide
| λabs | 650 nm |
|---|---|
| λem | 667 nm |
| Extinction Coefficient (ε) | 17600 M-1cm-1 |
| Quantum Yield (φ) | 0.53 |
Plan Your Experiments
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