MQAE

Name: MQAE
Brand: Tocris Bioscience
SKU: 7856

Tocris Bioscience, part of Bio-Techne | Catalog # 7856

COA / SDS

Fluorescent Cl- indicator; membrane permeable

Key Product Details

Description:	Fluorescent Cl^- indicator; membrane permeable
Chemical Name:	1-(2-Ethoxy-2-oxoethyl)-6-methoxyquinolin-1-ium bromide
Purity:	≥95% (HPLC)
Molecular Weight:	326.19

View Full Technical Details for MQAE

Technical Data
Citations

Biological Activity for MQAE

MQAE is a membrane-permeable intracellular chloride ion (Cl^-) indicator. The fluorescence intensity of MQAE decreases proportionally as Cl^- ions increase. Excitation/emission maxima (λ) are 355 nm and 460 nm respectively. Can be used in fluorescence microscopy and flow cytometry, suitable for use in vivo and in vitro.

Optical Data for MQAE

λ_abs	355 nm
λ_em	460 nm
Extinction Coefficient (ε)	4850 M^-1cm^-1
Cell Permeable	Yes
pH Sensitivity	Insensitive
Application	Confocal imaging, Flow cytometry

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for MQAE

M. Wt	326.19
Formula	C₁₄H₁₆BrNO₃
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	162558-52-3
PubChem ID	2762651
InChi Key	DSLLHVISNOIYHR-UHFFFAOYSA-M
Smiles	O=C(C[N+]1=CC=CC2=CC(OC)=CC=C21)OCC.[Br-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for MQAE

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	3.26	10
	water	3.26	10

Preparing Stock Solutions for MQAE

The following data is based on the product molecular weight 326.19.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	30.66 mL	153.28 mL	306.57 mL
0.5 mM	6.13 mL	30.66 mL	61.31 mL
1 mM	3.07 mL	15.33 mL	30.66 mL
5 mM	0.61 mL	3.07 mL	6.13 mL

Molarity Calculator

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Reconstitution Calculator

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References for MQAE

References are publications that support the biological activity of the product.

Wan An ATP-regulated ion transport nanosystem for homeostatic perturbation therapy and sensitizing photodynamic therapy by autophagy inhibition of tumors. ACS Cent.Sci. 2019 PMID: 30834321
Verkman Synthesis and characterization of improved chloride-sensitive fluorescent indicators for biological applications. Anal.Biochem. 1989 PMID: 2751097