NPE-caged-HPTS

Name: NPE-caged-HPTS
Brand: Tocris Bioscience
SKU: 2919

Tocris Bioscience, part of Bio-Techne | Catalog # 2919

COA / SDS

Caged fluorescent pH indicator

Discontinued Product

2919 has been discontinued. View all Ion Indicators products.

Key Product Details

Description:	Caged fluorescent pH indicator
Chemical Name:	8-Hydroxypyrene-1,3,6-tris-sulfonic acid-8-1-(2-nitrophenyl)ethyl ether
Purity:	≥97% (HPLC)
Molecular Weight:	673.53

View Full Technical Details for NPE-caged-HPTS

Technical Data
Citations

Biological Activity for NPE-caged-HPTS

NPE-caged-HPTS is a caged fluorescent pH indicator. Rapidly releases the fluorophore HPTS (pyranine) (pK_a 7.25) upon two-photon excitation (>3000 s^-1). Routinely used for photolysis calibration. Suitable for use in small-compartment diffusion studies.

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Technical Data for NPE-caged-HPTS

M. Wt	673.53
Formula	C₂₄H₁₄NO₁₂Na₃S₃
Storage	Store at -20°C
Purity	≥97% (HPLC)
CAS Number	223759-19-1
PubChem ID	56972217
InChi Key	CRGABPHUJQWBOR-UHFFFAOYSA-K
Smiles	[Na+].[Na+].[Na+].CC(OC1=C2C=CC3=C4C(C=CC(=C24)C(=C1)S([O-])(=O)=O)=C(C=C3S([O-])(=O)=O)S([O-])(=O)=O)C1=CC=CC=C1[N+]([O-])=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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References for NPE-caged-HPTS

References are publications that support the biological activity of the product.

Canepari Photochemical and pharmacological evaluation of 7-nitroindolinyl-and 4-methoxy-7-nitroindolinyl-amino acids as novel, fast caged neurotransmitters. J.Neurosci.Methods. 2001 PMID: 11640955
Soler-Llavina Synapse-specific plasticity and compartmentalized signaling in cerebellar stellate cells. Nat.Neurosci. 2006 PMID: 16680164
Trigo Laser photolysis of caged compounds at 405 nm: photochemical advantages, localisation, phototoxicity and methods for calibration. J.Neurosci.Methods 2009 PMID: 19427524
Bloodgood and Sabatini Neuronal activity regulates diffusion across the neck of dendritic spines. Science 2005 PMID: 16272125
Kiskin The efficiency of two-photon photolysis of a "caged " fluorophore, o-1-(2-nitrophenyl)ethylpyranine, in relation to photodamage of synaptic terminals. Eur.Biophys.J. 2002 PMID: 11908850
Jasuja Chemotactic responses of Escherichia coli to small jumps of photoreleased L-aspartate. Biophys.J. 1999 PMID: 10049350
Willoughby Comparisons of simultaneous pH measurements made with 8-hydroxypyrene-1,3,6-trisulphonic acid (HPTS) and pH-sensitive microelectrodes in snail neurones. Pflugers Arch. 1998 PMID: 9683736