NR12S
Tocris Bioscience, part of Bio-Techne | Catalog # 7509
Key Product Details
| Description: | Fluorogenic lipid membrane dye. Used for: staining lipid at the outer leaflet of vesicles and cell membranes, suitable for live cell imaging. Application: fluorescence microscopy |
| Chemical Name: | 3-((3-((9-(Diethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl)oxy)propyl)(dodecyl)(methyl)ammonio)propane-1-sulfonate |
| Purity: | ≥95% (HPLC) |
| Molecular Weight: | 695.96 |
Biological Activity for NR12S
Key information: NR12S is a fluorogenic lipid membrane dye that stains the outer leaflet of lipid vesicles and cell membranes. Suitable for live cell imaging.
Used for: monitoring cholesterol and lipid order selectively at the outer leaflet of cell membranes.
Application: fluorescence microscopy.
Properties and Photophysical Data: NR12S is a conjugation of the Nile Red fluorophore with a long alkyl chain and a zwitterionic group, allowing strong interactions with the lipid membranes. Excitation and emission maxima (λ) are 554 nm and 627 nm in DMSO, respectively; quantum yield = 0.55.
Optical Data for NR12S
| Emission Color | Orange |
|---|---|
| λabs | 554 nm |
| λem | 627 nm |
| Quantum Yield (φ) | 0.55 |
| Closest Laser line | 532 nm |
| Application | Monitoring of Lipid Dynamics in Living Cells, Fluorescence Microscopy |
| Alternative For | Nile Red |
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