O-Propargyl-puromycin

Name: O -Propargyl-puromycin
Brand: Tocris Bioscience
SKU: 7391

Tocris Bioscience, part of Bio-Techne | Catalog # 7391

Citations (4)

COA / SDS

Puromycin analog for labeling newly synthesized proteins

Key Product Details

Description:	Puromycin analog for labeling newly synthesized proteins
Chemical Name:	3'-[[(2S)-2-Amino-1-oxo-3-[4-(2-propyn-1-yloxy)phenyl]propyl]amino]-3'-deoxy-N,N-dimethyladenosine
Purity:	≥97% (HPLC)
Molecular Weight:	495.54

View Full Technical Details for O-Propargyl-puromycin

Technical Data
Citations

Biological Activity for O-Propargyl-puromycin

O-Propargyl-puromycin (OP-puro) is a puromycin analog that is incorporated into nascent polypeptides. It is used for the labeling of newly synthesized proteins in cells and whole animals in vivo. O-Propargyl-puromycin can be click-conjugated to fluorescent azides for visualization by fluoresence microscopy. The product does not require methionine-free conditions. It can also be used in flow cytometry.

Optical Data for O-Propargyl-puromycin

λ_em	275 nm
Reactivity	Click
Application	Fluorescence Microscopy, Flow Cytometry

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for O-Propargyl-puromycin

M. Wt	495.54
Formula	C₂₄H₂₉N₇O₅
Storage	Store at -20°C
Purity	≥97% (HPLC)
CAS Number	1416561-90-4
PubChem ID	71576433
InChi Key	JXBIGWQNNSJLQK-IYRMOJGWSA-N
Smiles	O[C@@H]1[C@H](NC([C@@H](N)CC2=CC=C(OCC#C)C=C2)=O)[C@@H](CO)O[C@H]1N3C4=NC=NC(N(C)C)=C4N=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for O-Propargyl-puromycin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	49.55	100

Preparing Stock Solutions for O-Propargyl-puromycin

The following data is based on the product molecular weight 495.54.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.02 mL	10.09 mL	20.18 mL
5 mM	0.40 mL	2.02 mL	4.04 mL
10 mM	0.20 mL	1.01 mL	2.02 mL
50 mM	0.04 mL	0.20 mL	0.40 mL

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References for O-Propargyl-puromycin

References are publications that support the biological activity of the product.

Morral Zonation of ribosomal DNA transcription defines a stem cell hierarchy in colorectal cancer. Cell Stem Cell 2020 PMID: 32396863
Liu Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. Proc.Natl.Acad.Sci.USA 2012 PMID: 22160674