PARPi-FL

Name: PARPi-FL
Brand: Tocris Bioscience
SKU: 6461

Tocris Bioscience, part of Bio-Techne | Catalog # 6461

Citations (4)

COA / SDS

Potent fluorescent PARP inhibitor; cell permeable

Key Product Details

Description:	Potent fluorescent PARP inhibitor; cell permeable
Alternative Names:	AZD2281-BDY FL,Olaparib-BDY FL
Chemical Name:	(T-4)-[4-[[3-[[4-[3-[5-[(3,5-Dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-1H-pyrrol-2-yl-κN-1-oxopropyl]-1-piperazinyl]carbonyl]-4-fluorophenyl]methyl]-1(2H)-phthalazinonato]difluoroboron
Purity:	≥90% (HPLC)
Molecular Weight:	640.46

View Full Technical Details for PARPi-FL

Technical Data
Citations

Biological Activity for PARPi-FL

PARPi-FL is a fluorescent potent PARP inhibitor (IC₅₀ = 12 nM). Composed of a PARP inhibitor conjugated to BDY FL, SE (Cat. No. 5465). Colocalizes with PARP immunostaining in multiple cancer cell lines in vivo and accumulates in tumor cells and tumor associated macrophages. Enables high temporal and subcellular spatial resolution of drug distribution. Emission max = 525 nm. Cell permeable.

Optical Data for PARPi-FL

Emission Color	Green
λ_abs	502 nm
λ_em	510 nm

Plan Your Experiments

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Technical Data for PARPi-FL

M. Wt	640.46
Formula	C₃₄H₃₂BF₃N₆O₃
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	1380359-84-1
PubChem ID	70697686
InChi Key	IGUTVNUEFKPBGK-UHFFFAOYSA-N
Smiles	CC1=CC(C)=[N+]2C1=CC3=CC=C(N3[B-]2(F)F)CCC(N4CCN(C(C5=CC(CC6=NNC(C7=CC=CC=C67)=O)=CC=C5F)=O)CC4)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PARPi-FL

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	6.4	10

Preparing Stock Solutions for PARPi-FL

The following data is based on the product molecular weight 640.46.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	15.61 mL	78.07 mL	156.14 mL
0.5 mM	3.12 mL	15.61 mL	31.23 mL
1 mM	1.56 mL	7.81 mL	15.61 mL
5 mM	0.31 mL	1.56 mL	3.12 mL

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References for PARPi-FL

References are publications that support the biological activity of the product.

Thurber Effect of small-molecule modification on single-cell pharmacokinetics of PARP inhibitors. Mol.Cancer Ther. 2014 PMID: 24552776
Dubach Quantitating drug-target engagement in single cells in vitro and in vivo. Nat.Chem.Biol. 2017 PMID: 27918558
Thurber Single-cell and subcellular pharmacokinetic imaging allows insight into drug action in vivo. Nat.Commun. 2013 PMID: 23422672