RADA

Name: RADA
Brand: Tocris Bioscience
SKU: 6649

Tocris Bioscience, part of Bio-Techne | Catalog # 6649

Citations (6)

COA / SDS

Orange-red TAMRA-based fluorescent D-amino acid for labeling peptidoglycans in live bacteria

Key Product Details

Purity:

≥98%

View Full Technical Details for RADA

Technical Data
Citations

Biological Activity for RADA

RADA is an orange-red TAMRA-based fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Suitable for prolonged exposure due to red-shift photophysical properties. Suitable for dSTORM and reSTORM. Displays good water solubility, photostability and thermostability. Excitation/emission λ ~554/580 nm.

This product is supplied as a TFA salt, the stoichiometry of which is batch dependant. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.

Scientific Data Examples for RADA

	Application of RADA. Using FDAAs RADA and HADA (Catalog # 6647) to study peptidoglycan enzyme and substrate distribution, and the role of polar cell growth and peptidoglycan segregation over time. Image shows peptidoglycan segregation over time. Left to right - 2 mins HADA pulse (cyan), 45 min outgrowth, followed by 2 min RADA chase (magenta) in WT Msm cells. New cell wall (magenta), oldest cell wall (cyan). Scale: 5 μm.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for RADA

Formula	C₂₈H₂₈N₄O₆
Storage	Store at -20°C
Purity	≥98%

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for RADA

Solubility

Soluble Soluble to 100 mM in DMSO

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for RADA

References are publications that support the biological activity of the product.

Hsu et al. Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem.Sci., 2017;8:6313 PMID: 28989665
García-Heredia et al. Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. Elife, 2018;7:pii: e37243. PMID: 30198841
Fenton et al. Erratum: CozE is a member of the MreCD complex that directs cell elongation in Streptococcus pneumoniae. Nat.Microbiol., 2017;2:17011 PMID: 28112724