Rainbow Calibration Particle Set (8 peaks - EAL01) - EuroFlow(TM)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00506
Key Product Details
Conjugate
Unconjugated
Applications
Flow Cytometry, Microscopy
Product Specifications
Description
Rainbow Calibration Particles are a mixture of fluorophore incorporated particles with 8 different fluorescent intensities that are used for calibrating flow cytometers:
Particle size: 3.0 - 3.4 micron
Particle material: polystyrene
Uses: Long term performance tracking or routine calibration of flow cytometers, specific for following the EuroFlow(TM) standardization guidelines. Routine alignment and calibration in fluorescence and confocal fluorescence microscopy.
- Excite particles at any wavelength from 365 to 650 nm.
- Calibrate multiple channels with the same set of particles.
- Enable non-spectral matching to common fluorophores such as FITC, PE, PE-Cy5.
- Deliver durable performance since fluorophores are encased within particles, not on the surface.
- Remain stable for several years.
- Packaged in a dropper bottle for easy dispensing and storage.
Particle size: 3.0 - 3.4 micron
Particle material: polystyrene
Uses: Long term performance tracking or routine calibration of flow cytometers, specific for following the EuroFlow(TM) standardization guidelines. Routine alignment and calibration in fluorescence and confocal fluorescence microscopy.
Application Notes
Shake vigorously or vortex briefly before use. Inclusion of a small amount of detergent in the diluent will help increase the number of singles. Diluted particles can be stored at 4C for future use.
Scientific Data Images for Rainbow Calibration Particle Set (8 peaks - EAL01) - EuroFlow(TM)
Rainbow Calibration Particle Set (8 peaks - EAL01) [NBP3-00506] - Dot Plot and histograms for NBP3-00506 on a BD Biosciences LSRFortessa(TM) X-20 using assigned target values.
Formulation, Preparation and Storage
NBP3-00506
| Formulation | Deionized water, 0.01% NP-40 |
| Preservative | 0.02% Sodium Azide |
| Concentration | Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C in the dark. Do not freeze. |
Background: Rainbow Calibration Particle Set (8 peaks - EAL01) - EuroFlow(TM)
The relative number of fluorophores can been determined for every emission peak of the rainbow calibration particles in FL1 (FITC, MEFL), FL2 (RPE, MEPE), FL3 (RPE-Cy5, MEPCY) and FL4 (APC, MEAP) channels in order to plot the calibration graph. The calibration graph is used to check the linearity (dynamic range) of the photomultiplier tube (PMT) in each channel. Additionally, the relative number of fluorophores can be cross calibrated with cells or particles stained with a known number of spectral matching fluorophores such as FITC, PE, RPE-Cy5 to estimate the number of fluorophores on stained cells.
Rainbow Calibration Particle Set (8 peaks - EAL01) are used in studies following EuroFlow(TM) standard guidelines for PMT characterization, to set target mean fluorescence intensities (MFI) values and for daily performance tracking of flow cytometers. They provide target MFIs for the standardization of an 8-color flow cytometric immunophenotyping panel of normal and malignant leukocytes in bone marrow and blood (1).
References
1. Kalina, T., Flores-Montero, J., van der Velden V.H.J., Martin-Ayuso, M., Bottcher, S., Ritgen, M., Almeida, J., Lhermitte,L., Asnafi, V., Mendonca, A., de Tute, R., Cullen, M., Sedek, L., Vidriales, M.B., Perez, J.J., te Marvelde, J.G., Mejstrikova, E., Hrusak, O., Szczepanski, T., van Dongen, J.J.M., and Orfao A.. (2012). EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. 26(9):1986-2010. https://doi.org/10.1038/leu.2012.122
EuroFlow(TM) is a trademark of European Scientific foundation for Laboratory Hemato Oncology (ESLHO).
Alternate Names
Calibration Beads, Flow Cytometry Calibration, Rainbow Calibration Beads
Additional Rainbow Calibration Particle Set (8 peaks - EAL01) - EuroFlow(TM) Products
Product Documents for Rainbow Calibration Particle Set (8 peaks - EAL01) - EuroFlow(TM)
Product Specific Notices for Rainbow Calibration Particle Set (8 peaks - EAL01) - EuroFlow(TM)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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