sBADA

Name: sBADA
Brand: Tocris Bioscience
SKU: 7860

Tocris Bioscience, part of Bio-Techne | Catalog # 7860

Citations (3)

COA / SDS

Green fluorescent D-amino acid for labeling peptidoglycans in bacteria

Key Product Details

Description:	Green fluorescent D-amino acid for labeling peptidoglycans in bacteria
Chemical Name:	(R)-2-Amino-3-(3-(5,5-difluoro-7,9-dimethyl-2-sulfo-5H-4λ⁴,5λ⁴-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-3-yl)propanamido)propanoic acid
Purity:	≥95% (HPLC)
Molecular Weight:	458.24

View Full Technical Details for sBADA

Technical Data
Citations

Biological Activity for sBADA

sBADA is a green sulfonated BODIPY-FL 3-amino-D-alanine (sBADA) or fluorescent D-amino acid (FFDA). Labels peptidoglycans in live bacteria. Sulfonated form of BADA with increased hydrophilicity and thermostability. Excitation/emission λ ~490/510 nm.

Optical Data for sBADA

Emission Color	Green
λ_abs	490 nm
λ_em	510 nm
Extinction Coefficient (ε)	49000 M^-1cm^-1
Application	Fluorescence Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for sBADA

M. Wt	458.24
Formula	C₁₇H₂₁BF₂N₄O₆S
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	2376838-11-6
PubChem ID	138991774
InChi Key	OLMGTLBVHKBMSH-GFCCVEGCSA-N
Smiles	CC(C=C1C)=C2N1[B-](F)(F)[N+]3=C(CCC(NC[C@@H](N)C(O)=O)=O)C(S(=O)(O)=O)=CC3=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for sBADA

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	4.58	10

Preparing Stock Solutions for sBADA

The following data is based on the product molecular weight 458.24.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	21.82 mL	109.11 mL	218.23 mL
0.5 mM	4.36 mL	21.82 mL	43.65 mL
1 mM	2.18 mL	10.91 mL	21.82 mL
5 mM	0.44 mL	2.18 mL	4.36 mL

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References for sBADA

References are publications that support the biological activity of the product.

Hsu Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem.Sci. 2017 PMID: 28989665