Tricyclic cytosine tC

Name: Tricyclic cytosine tC
Brand: Tocris Bioscience
SKU: 7814

Tocris Bioscience, part of Bio-Techne | Catalog # 7814

COA / SDS

Fluorescent tricyclic cytosine analog for RNA imaging

Key Product Details

Description:	Fluorescent tricyclic cytosine analog for RNA imaging
Chemical Name:	3-β-D-Ribofuranosyl-1H-pyrimido[5,4-b][1,4]benzothiazin-2(3H)-one
Purity:	≥95% (HPLC)
Molecular Weight:	349.36

View Full Technical Details for Tricyclic cytosine tC

Technical Data
Citations

Biological Activity for Tricyclic cytosine tC

Tricyclic cytosine tC is a fluorescent cytosine analog for imaging RNA in living cells. Enables the investigation of RNA synthesis, degradation, and trafficking at single-cell resolution when combined with confocal imaging. Excitation/emission maxima (λ) = 375/505 nm; extinction coefficient = 4000 M^-1 cm^-1; quantum yield = 0.2.

Optical Data for Tricyclic cytosine tC

λ_abs	375 nm
λ_em	505 nm
Extinction Coefficient (ε)	4000 M^-1cm^-1
Quantum Yield (φ)	0.2
Application	Confocal Imaging

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for Tricyclic cytosine tC

M. Wt	349.36
Formula	C₁₅H₁₅N₃O₅S
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	1174063-74-1
InChi Key	SRXXFIYNNTZZBB-LHNIVKCTSA-N
Smiles	O[C@H]1[C@H](N2C(N=C3C(SC4=CC=CC=C4N3)=C2)=O)O[C@@H]([C@H]1O)CO

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Tricyclic cytosine tC

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	34.94	100

Preparing Stock Solutions for Tricyclic cytosine tC

The following data is based on the product molecular weight 349.36.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.86 mL	14.31 mL	28.62 mL
5 mM	0.57 mL	2.86 mL	5.72 mL
10 mM	0.29 mL	1.43 mL	2.86 mL
50 mM	0.06 mL	0.29 mL	0.57 mL

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Reconstitution Calculator

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References for Tricyclic cytosine tC

References are publications that support the biological activity of the product.

Stengel Incorporation of the fluorescent ribonucleotide analogue tCTP by T7 RNA polymerase. Anal.Chem. 2010 PMID: 20067253
Sandin Fluorescent properties of DNA base analogue tC upon incorporation into DNA--negligible influence of neighbouring bases on fluorescence quantum yield. Nucleic Acids Res. 2005 PMID: 16147985
Wang Live-cell RNA imaging with metabolically incorporated fluorescent nucleosides. J.Am.Chem.Soc. 2022 PMID: 35930766