TSA Vivid™ Fluorophore Kit 520
Tocris Bioscience, part of Bio-Techne | Catalog # 7523
Key Product Details
| Description: | Signal amplification kit for use in ISH, ICC and IHC |
Biological Activity for TSA Vivid™ Fluorophore Kit 520
TSA Vivid™ reagents are exceptionally bright dyes that offer an effective way to boost signal intensity and detect low-abundance targets in spatial biology applications. They are designed for use in ISH, IHC and ICC experiments where in situ detection of target protein or nucleic acid sequences is required. TSA Vivid reagents have been specifically optimized to deliver exceptional signal-to-noise in the RNAscope™ Multiplex Fluorescent v2 Assay for visualization of gene expression at the single cell level. They are suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining.
The basis for signal amplification comes from the specific and high-density deposition of TSA Vivid dyes adjacent to a horseradish peroxidase (HRP)-labeled probe in a process termed Tyramide Signal Amplification (TSA) or Catalyzed Reporter Deposition (CARD).
Excitation maximum = 494 nm; emission maximum = 517 nm
Please see the product datasheet or protocol for information on product use.
TSA Vivid is a trademark of Bio-Techne Corp.
Optical Data
 | Emission Color | Green |
| λabs | 494 nm | |
| λem | 517 nm |
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Application of TSA Vivid dye 520 in RNAscope Multiplex Fluorescent v2 Assay RNAscope™ Multiplex Fluorescent v2 Assay on HeLa cells with the leading competitor dye (left) and TSA Vivid dye 520 (right). Dye used at 1:1500 dilution. Marker shown is Polr2a in green. Nuclei are counterstained with DAPI (blue, Cat. No. 5748). |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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