Ultra Rainbow Calibration ERF Particle Set (6 peaks)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11817
Key Product Details
Applications
Flow Cytometry, Microscopy
Product Summary for Ultra Rainbow Calibration ERF Particle Set (6 peaks)
Ultra Rainbow Quantitative Particle Kit
Contains 6 intensities of Ultra Rainbow Fluorescent Beads with NIST assigned ERF (Equivalent Number of Reference Fluorophores) values based on a published procedure using NIST SRM 1934 and a calibrated laserbased CCD fluorimeter. Provides microsphere reference standards for fluorescein isothiocyanate (FITC), phycoerythrin (PE), allophycocyanin (APC) and Pacific Blue (PB) fluorescent channels using four different NIST reference fluorophores; fluorescein, Nile Red, APC and Coumarin 30. Used during flow cytometry standardardization and calibration measurements for the further advancement of biological and clinical applications. Particle size: 3.5 -3.9 micron
Size: 6 x 2 mL
Contains 6 intensities of Ultra Rainbow Fluorescent Beads with NIST assigned ERF (Equivalent Number of Reference Fluorophores) values based on a published procedure using NIST SRM 1934 and a calibrated laserbased CCD fluorimeter. Provides microsphere reference standards for fluorescein isothiocyanate (FITC), phycoerythrin (PE), allophycocyanin (APC) and Pacific Blue (PB) fluorescent channels using four different NIST reference fluorophores; fluorescein, Nile Red, APC and Coumarin 30. Used during flow cytometry standardardization and calibration measurements for the further advancement of biological and clinical applications. Particle size: 3.5 -3.9 micron
Size: 6 x 2 mL
Scientific Data Images for Ultra Rainbow Calibration ERF Particle Set (6 peaks)
Ultra Rainbow Calibration ERF Particle Set (6 peaks) [NBP3-11817]
Ultra Rainbow Calibration ERF Particle Set (6 peaks) [NBP3-11817]
Formulation, Preparation, and Storage
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C in the dark. Do not freeze.
Background: Ultra Rainbow Calibration ERF Particle Set (6 peaks)
The relative number of fluorophores can been determined for every emission peak of the rainbow calibration particles in FL1 (FITC, MEFL), FL2 (RPE, MEPE), FL3 (RPE-Cy5, MEPCY) and FL4 (APC, MEAP) channels in order to plot the calibration graph. The calibration graph is used to check the linearity (dynamic range) of the photomultiplier tube (PMT) in each channel. Additionally, the relative number of fluorophores can be cross calibrated with cells or particles stained with a known number of spectral matching fluorophores such as FITC, PE, RPE-Cy5 to estimate the number of fluorophores on stained cells.
Additional Ultra Rainbow Calibration ERF Particle Set (6 peaks) Products
Product Documents for Ultra Rainbow Calibration ERF Particle Set (6 peaks)
Product Specific Notices for Ultra Rainbow Calibration ERF Particle Set (6 peaks)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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