Mouse IgG F(ab)2 Isotype Control
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97018
![SDS-PAGE: Mouse IgG F(ab)2 Isotype Control [NBP1-97018]](https://resources.bio-techne.com/images/products/Mouse-IgG-F-ab-2-Isotype-Control-SDS-Page-NBP1-97018-img0002.jpg "SDS-PAGE: Mouse IgG F(ab)2 Isotype Control [NBP1-97018]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Immunohistochemistry, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Mouse IgG
Format
Purified
Concentration
LYOPH mg/ml
Product Specifications
Specificity
No reaction was observed against anti-Mouse IgG F(c) or anti-Pepsin.
Host
Mouse
Isotype
IgG
Description
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, anti-Mouse IgG and anti-Mouse IgG F(ab')2
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Mouse IgG F(ab)2 Isotype Control
SDS-PAGE: Mouse IgG F(ab)2 Isotype Control [NBP1-97018]
SDS-Page: Mouse IgG F(ab)2 Isotype Control [NBP1-97018] - SDS-PAGE of Mouse IgG F(ab')2 Fragment. Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab')2 Fragment. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab')2 at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.Applications for Mouse IgG F(ab)2 Isotype Control
Application
Recommended Usage
Immunohistochemistry
1:10 - 1:500
Application Notes
This product has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Purified
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG F(ab)2
Additional IgG F(ab)2 Products
Product Documents for Mouse IgG F(ab)2 Isotype Control
Product Specific Notices for Mouse IgG F(ab)2 Isotype Control
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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